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PMID: 378943 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Characterization of Azotobacter vinelandii deoxyribonucleic acid and folded chromosomes.

Journal of bacteriology ·Vol. 138 ·No. 3 ·1979-06-00 ·Pages 871-7

Sadoff HL, Shimel B, Ellis S

Abstract

The properties of Azotobacter vinelandii deoxyribonucleic acid (DNA) and folded chromosomes were studied and compared to those of Escherichia coli as a standard. Based on melting temperature and buoyant density measurements, the guanosine + cytosine content of purified A. vinelandii DNA was 65%, whereas that of E. coli DNA was 50%. The results of renaturation studies showed that the unique DNA sequence lengths of the two organisms were similar with Cot1/2 values of 7.3 +/- 0.4 mol.s/liter and 7.5 +/- 0.3 mol.s/liter, respectively, for A. vinelandii and E. coli. Folded chromosomes of A. vinelandii sedimented in a centrifugal field at a rate identical to those derived from E. coli, 1,600 to 1,700S. Based on the DNA content per cell and the mass of a single genome, A. vinelandii contains at least 40 chromosomes per cell.

MeSH Terms
Azotobacter/analysis Base Sequence Chromosomes, Bacterial/analysis Cytosine/analysis DNA, Bacterial/analysis Escherichia coli/analysis Guanosine/analysis Hot Temperature Nucleic Acid Denaturation Nucleic Acid Renaturation Species Specificity
Chemicals
DNA, Bacterial Guanosine Cytosine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sadoff H L
Shimel B
Ellis S
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24 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.138.3.871-877.1979
Published
1979-06-00
Pages
871-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC218116
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.138.3.871-877.1979 PMC Full Text View journal details
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