Mutant of Azotobacter vinelandii that hyperproduces nitrogenase component II.

Journal of bacteriology ·Vol. 117 ·No. 2 ·1974-02-00 ·Pages 917-9

Shah VK, Davis LC, Stieghorst M, Brill WJ

Abstract

A mutant strain of Azotobacter vinelandii that is unable to fix N(2) produces high levels of nitrogenase component II. Activities of revertants from this mutant strain indicate that a single genetic lesion is responsible for both hyperproduction of component II and the inability to produce component I.

MeSH Terms

Azotobacter/enzymology,metabolism Bacterial Proteins/biosynthesis Cell-Free System Culture Media Genes Immunodiffusion Models, Biological Mutation Nitrogenase/biosynthesis Quaternary Ammonium Compounds/metabolism Spectrophotometry

Chemicals

Bacterial Proteins Culture Media Quaternary Ammonium Compounds Nitrogenase

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Shah V K

Davis L C

Stieghorst M

Brill W J

References (7)

7 references, click to expand

The nitrogenase system from Azotobacter: two-enzyme requirement for N2 reduction, ATP-dependent H2 evolution, and ATP hydrolysis.
Proc Natl Acad Sci U S A. 1966 Sep;56(3):979-86 PMID: 5230193
A quantitative study of a technique of double diffusion in agar.
J Immunol. 1956 Jul;77(1):52-60 PMID: 13346095
Nitrogenase. IV. Simple method of purification to homogeneity of nitrogenase components from Azotobacter vinelandii.
Biochim Biophys Acta. 1973 May 30;305(2):445-54 PMID: 4354875
Formation of the nitrogen-fixing enzyme system in Azotobacter vinelandii.
Can J Microbiol. 1968 Jan;14(1):25-31 PMID: 5644401
Mutants of Azotobacter vinelandii unable to fix nitrogen.
Biochim Biophys Acta. 1969 Jun 17;184(1):99-105 PMID: 5791118
Nitrogenase. I. Repression and derepression of the iron-molybdenum and iron proteins of nitrogenase in Azotobacter vinelandii.
Biochim Biophys Acta. 1972 Feb 28;256(2):498-511 PMID: 5016550
Nitrogenase. 3. Nitrogenaseless mutants of Azotobacter vinelandii: activities, cross-reactions and EPR spectra.
Biochim Biophys Acta. 1973 Jan 18;292(1):246-55 PMID: 4350172

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.117.2.917-919.1974

Published

1974-02-00

Pages

917-9

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC285593

Subset

External Links

PubMed source DOI: 10.1128/jb.117.2.917-919.1974 PMC Full Text View journal details

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Mutant of Azotobacter vinelandii that hyperproduces nitrogenase component II.

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