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PMID: 3785197 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Transcriptional and posttranscriptional regulation of exogenous human beta interferon gene in simian cells defective in interferon synthesis.

Molecular and cellular biology ·Vol. 6 ·No. 6 ·1986-06-00 ·Pages 2279-83

Mosca JD, Pitha PM

Abstract

We determined that the defect in beta interferon induction in Vero cells is due to the absence of the simian beta interferon (IFN-beta) gene. Nevertheless, the human IFN-beta gene or a hybrid gene, in which the human IFN-beta promoter-regulatory region directs expression of the chloramphenicol acetyltransferase gene (pIFN-CAT), could be induced in transfected Vero cells, and these cells also regulated IFN-beta mRNA (but not pIFN-CAT mRNA) posttranscriptionally. These results indicate that the instability in the human IFN-beta gene is coded for by the coding or 3'-end region of IFN-beta mRNA and that the human IFN-beta gene is regulated in Vero and human cells in an identical manner.

MeSH Terms
Animals Gene Expression Regulation Genes Humans Interferon Type I/genetics Promoter Regions, Genetic RNA, Messenger/metabolism Recombinant Fusion Proteins/biosynthesis Transcription, Genetic Vero Cells/physiology
Chemicals
Interferon Type I RNA, Messenger Recombinant Fusion Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mosca J D
Pitha P M
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32 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.6.6.2279-2283.1986
Published
1986-06-00
Pages
2279-83
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC367773
Subset
IM
Grants
NCI NIH HHS · 5P30CA06973-23 · United States
External Links
PubMed source DOI: 10.1128/mcb.6.6.2279-2283.1986 PMC Full Text View journal details
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