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PMID: 3782291 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A fractionated cell-free system for analysis of prophase nuclear disassembly.

The Journal of cell biology ·Vol. 103 ·No. 6 Pt 1 ·1986-12-00 ·Pages 2073-81

Suprynowicz FA, Gerace L

Abstract

We describe a cell-free system in which a postribosomal supernatant (s140) from metaphase Chinese hamster ovary (CHO) cells induces prophase-like changes in isolated CHO cell nuclei, including chromatin condensation, and nuclear envelope and lamina disassembly. These events are strongly promoted by gamma-S-ATP and an ATP-regenerating system, and do not take place with an s140 derived from G2-phase cells. The metaphase cell s140 also induces disassembly of an isolated nuclear lamina fraction that is depleted of membranes, chromatin, and nuclear pore complexes. Disassembly of the isolated lamina is accompanied by phosphorylation of the major lamina proteins (lamins A, B, and C) to levels characteristic of metaphase cells. Kinetic analysis of lamina depolymerization indicates that cooperativity may be involved in this process. The biochemical properties of in vitro lamina disassembly suggest that the activity that depolymerizes the lamina during mitosis is soluble in metaphase cells, and support the notion that this activity is a lamin protein kinase.

MeSH Terms
Animals Cell Fractionation Cell Line Cell Nucleus/ultrastructure Cell-Free System Cricetinae Cricetulus Female Kinetics Laminin/metabolism Metaphase Microscopy, Electron Ovary Phosphorylation Prophase
Chemicals
Laminin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Suprynowicz F A
Gerace L
References (35)
35 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.103.6.2073
Published
1986-12-00
Pages
2073-81
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2114623
Subset
IM
Grants
NIGMS NIH HHS · GM28521 · United States
External Links
PubMed source DOI: 10.1083/jcb.103.6.2073 PMC Full Text View journal details
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