Control of adenylate cyclase from secretory vesicle membranes by beta-adrenergic agents and nerve growth factor.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 3 ·1976-03-00 ·Pages 771-4

Nikodijevic O, Nikodijevic B, Zinder O, Yu MY, Guroff G, Pollard HB

Abstract

Adenylate cyclase [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1] activity of purified secretory vesicle membranes from the adrenal medulla is inhibited by I-isoproterenol and I-epinephrine, as well as by nerve growth factor (NGF). The effect of these agents was found to be dose-dependent and, in the case of the catecholamines, saturable. NGF was active at concentrations as low as 10(-8) M. Oxidized NGF was only minimally active, and insulin was completely inactive. Neither dopamine nor phenylephrine had activity. Inhibition of cyclase by either isoproterenol or epinephrine was blocked by I-propranolol, a specific beta-antagonist, but propranolol by itself had no effect on adenylate cyclase activity. The data indicate that the secretory vesicle membrane has beta-adrenergic receptors coupled to the adenylate cyclase. Propranolol was also found to block the NGF-induced inhibition of cyclase. We conclude that the granule membrane has beta-adrenergic receptors as well as NGF-reactive sites, and that the two may be functionally linked.

MeSH Terms

Adenylyl Cyclase Inhibitors Adenylyl Cyclases/metabolism Adrenal Medulla/enzymology,ultrastructure Adrenergic Agonists/pharmacology Animals Dose-Response Relationship, Drug Membranes/enzymology Nerve Growth Factors/pharmacology Organoids/enzymology Propranolol/pharmacology Receptors, Adrenergic

Chemicals

Adenylyl Cyclase Inhibitors Adrenergic Agonists Nerve Growth Factors Receptors, Adrenergic Propranolol Adenylyl Cyclases

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Nikodijevic O

Nikodijevic B

Zinder O

Yu M Y

Guroff G

Pollard H B

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23 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.73.3.771

Published

1976-03-00

Pages

771-4

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC336000

Subset

External Links

PubMed source DOI: 10.1073/pnas.73.3.771 PMC Full Text View journal details

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Control of adenylate cyclase from secretory vesicle membranes by beta-adrenergic agents and nerve growth factor.

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