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PMID: 370789 Published · ppublish English Journal Article

The interaction of RNA polymerase II from wheat with supercoiled and linear plasmid templates.

Nucleic acids research ·Vol. 6 ·No. 2 ·1979-02-00 ·Pages 507-23

Lilley DM, Houghton M

Abstract

Interactions between a plant RNA polymerase II and ColE1 based plasmid DNA templates have been studied. Gel electrophoresis indicates that the enzyme binds to both supercoiled and linear species. Using the totally double stranded pMB9/SmaI fragment it is shown that transcription of completely base paired DNA is ten-fold lower than that of denatured or supercoiled plasmid, and reflects the presence of fewer initiation sites. A small proportion of the transcript remains tightly bound to supercoiled templates. 3' oligodeoxycytidine extensions on pMB9/SmaI serve to promote transcription of the linear double stranded form. Using restriction kinetics it is shown that there is a small enhancement of polymerase binding at the pMB9 tetracycline promoter, but that the selectivity of binding at this locus is lower than for the natural bacterial polymerase.

MeSH Terms
DNA, Superhelical/metabolism DNA-Directed RNA Polymerases/metabolism Escherichia coli/enzymology Kinetics Plants/enzymology Plasmids RNA Polymerase II/metabolism Templates, Genetic Transcription, Genetic Triticum/enzymology
Chemicals
DNA, Superhelical RNA Polymerase II DNA-Directed RNA Polymerases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lilley D M
Houghton M
References (47)
47 references, click to expand
  1. RNA polymerase interaction at the promoter--operator region of the tryptophan operon of Escherichia coli and Salmonella typhimurium.
    J Mol Biol. 1978 May 15;121(2):153-77 PMID: 351193
  2. Transcription of polyoma virus DNA in vitro. III. Localization of calf thymus RNA polymerase II binding sites.
    J Mol Biol. 1978 Sep 5;124(1):87-96 PMID: 213608
  3. Transcription of specific adenovirus genes in isolated nuclei by exogenous RNA polymerases.
    J Biol Chem. 1977 Dec 10;252(23):8753-61 PMID: 925020
  4. Inhibition of transcription of supercoiled PM2 DNA by carbodiimide modification.
    Nucleic Acids Res. 1977 Jun;4(6):1713-26 PMID: 331264
  5. Transcription specificity of yeast RNA polymerase A. Highly specific transcription in vitro of the homologous ribosomal transcription units.
    Eur J Biochem. 1977 Oct 3;79(2):579-88 PMID: 923568
  6. On the initiation of mammalian RNA polymerase at single-strand breaks in DNA.
    Eur J Biochem. 1976 Nov 1;70(1):63-74 PMID: 1009933
  7. Transcription of polyoma virus DNA in vitro. II. Transcription of superhelical and linear polyoma DNA by RNA polymerase II.
    J Mol Biol. 1978 Sep 5;124(1):73-85 PMID: 213607
  8. DNA binding, condensing and unwinding properties of yeast RNase H1.
    J Biol Chem. 1977 Dec 25;252(24):8935-44 PMID: 200623
  9. Construction and characterization of new cloning vehicles. I. Ampicillin-resistant derivatives of the plasmid pMB9.
    Gene. 1977;2(2):75-93 PMID: 344136
  10. Active chromatin structure.
    Cell Biol Int Rep. 1978 Jan;2(1):1-10 PMID: 343927
  11. Isolation, characterization, and structure of the folded interphase genome of Drosophila melanogaster.
    Cell. 1976 Nov;9(3):393-407 PMID: 825231
  12. Transcription specificity of Xenopus laevis RNA polymerase A.
    FEBS Lett. 1974 Oct 15;47(2):304-6 PMID: 4473382
  13. The purification and properties of hen oviduct form B DNA-dependent RNA polymerase.
    Nucleic Acids Res. 1974 Feb;1(2):299-308 PMID: 4472377
  14. Effects of supercoiling on transcription from bacteriophage PM2 deoxyribonucleic acid.
    Biochemistry. 1974 Jul 16;13(15):3164-9 PMID: 4601430
  15. Role of deoxyribonucleic acid-ribonucleic acid hybrids in eukaryotes. Synthetic ribo- and deoxyribopolynucleotides as template for yeast ribonucleic acid polymerase B (or II).
    J Biol Chem. 1974 Sep 25;249(18):5978-83 PMID: 4607037
  16. Role of deoxyribonucleic acid-ribonucleic acid hybrids in eukaryotes. Study of the template requirements of yeast ribonucleic acid polymerases and nature of the ribonucleic acid product.
    J Biol Chem. 1974 Sep 25;249(18):5971-7 PMID: 4370349
  17. Interactions between twisted DNAs and enzymes: the effects of superhelical turns.
    J Mol Biol. 1974 Aug 25;87(4):797-816 PMID: 4279300
  18. Animal DNA-dependent RNA polymerases. Studies on the binding of mammalian RNA polymerases AI and B to Simian virus 40 DNA.
    Eur J Biochem. 1974 Jan 16;41(2):397-411 PMID: 4361419
  19. Animal DNA-dependent RNA polymerases. Analysis of the RNAs synthesized on Simian virus 40 superhelical DNA by mammalian RNA polymerases AI and B.
    Eur J Biochem. 1974 Jan 16;41(2):379-95 PMID: 4361418
  20. Animal DNA-dependent RNA polymerases. Studies on the reaction parameters of transcription in vitro of Simian virus 40 DNA by mammalian RNA polymerases AI and B.
    Eur J Biochem. 1974 Jan 16;41(2):367-78 PMID: 4361417
  21. Transcription of Xenopus chromatin by homologous ribonucleic acid polymerase: aberrant synthesis of ribosomal and 5S ribonucleic acid.
    Biochemistry. 1974 Apr 23;13(9):1896-9 PMID: 4857734
  22. Effect of circularity and superhelicity on transcription from bacteriophagelambda DNA.
    Proc Natl Acad Sci U S A. 1973 Nov;70(11):3077-81 PMID: 4594034
  23. Template specificity of eucaryotic DNA-dependent RNA polymerases. Effect of DNA structure and integrity.
    Eur J Biochem. 1974 Mar 1;42(2):567-79 PMID: 4364253
  24. Animal DNA-dependent RNA polymerases. 4. Studies on inhibition by rifamycin derivatives.
    Eur J Biochem. 1972 Jul 13;28(2):291-300 PMID: 4560736
  25. Inhibition by derivatives of rifamycin of soluble ribonucleic acid polymerase from rat liver.
    Biochem J. 1972 Aug;128(5):1361-4 PMID: 4643707
  26. Integrity of the DNA template, a prerequisite for the faithful transcription of Xenopus rDNA in vitro.
    Eur J Biochem. 1972 Sep 25;29(3):489-99 PMID: 5083507
  27. Stimulation by cyclic adenosine monophosphate of plasmid deoxyribonucleic acid replication and catabolite repression of the plasmid deoxyribonucleic acid-protein relaxation complex.
    J Bacteriol. 1973 May;114(2):577-91 PMID: 4350343
  28. Role of DNA-RNA hybrids in eukaryotes. Purification and properties of yeast RNA polymerase B.
    Eur J Biochem. 1973 Apr 2;34(1):41-52 PMID: 4573680
  29. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.
    Biochemistry. 1973 Jul 31;12(16):3055-63 PMID: 4354250
  30. Cleavage of circular, superhelical simian virus 40 DNA to a linear duplex by S1 nuclease.
    J Virol. 1973 Dec;12(6):1303-13 PMID: 4357509
  31. Early and late helix-coil transitions in closed circular DNA. The number of superhelical turns in polyoma DNA.
    J Mol Biol. 1968 Apr 14;33(1):173-97 PMID: 4296518
  32. Introduction of interrupted secondary structure in supercoiled DNA as a function of superhelix density: consideration of hairpin structures in superhelical DNA.
    J Virol. 1976 Apr;18(1):195-204 PMID: 1255870
  33. Attachment of nascent RNA molecules to superhelical DNA.
    J Mol Biol. 1975 Nov 5;98(3):565-79 PMID: 811809
  34. A new method for the large-scale purification of wheat germ DNA-dependent RNA polymerase II.
    Biochemistry. 1975 Oct 21;14(21):4639-45 PMID: 1182107
  35. A procedure for the rapid, large-scall purification of Escherichia coli DNA-dependent RNA polymerase involving Polymin P precipitation and DNA-cellulose chromatography.
    Biochemistry. 1975 Oct 21;14(21):4634-8 PMID: 1101952
  36. Eukaryotic nuclear RNA polymerases.
    Annu Rev Biochem. 1975;44:613-38 PMID: 1094919
  37. A system for mapping DNA sequences in the chromosomes of Drosophila melanogaster.
    Cell. 1974 Dec;3(4):315-25 PMID: 4216403
  38. On the structure of the folded chromosome of Escherichia coli.
    J Mol Biol. 1972 Nov 14;71(2):127-47 PMID: 4564477
  39. Light-scattering studies on supercoil unwinding.
    Biochem J. 1973 Jun;133(2):209-26 PMID: 4723772
  40. Effect of DNA length on the free energy of binding of an unwinding ligand to a supercoiled DNA.
    J Mol Biol. 1972 May 14;66(2):307-9 PMID: 5038457
  41. A restriction enzyme from Hemophilus influenzae. I. Purification and general properties.
    J Mol Biol. 1970 Jul 28;51(2):379-91 PMID: 5312500
  42. Conformational analysis of deoxyribonucleic acid from PM2 bacteriophage. The effect of size on supercoil shape.
    Biochem J. 1976 Apr 1;155(1):101-5 PMID: 938468
  43. Supercoils in human DNA.
    J Cell Sci. 1975 Nov;19(2):261-79 PMID: 1202042
  44. Supercoiled DNA folded by non-histone proteins in cultured mammalian cells.
    Nature. 1975 Dec 4;258(5534):445-7 PMID: 1196378
  45. DNA of bacteriophage PM2: a closed circular double-stranded molecule.
    Proc Natl Acad Sci U S A. 1969 Aug;63(4):1164-8 PMID: 5260915
  46. A DNA-RNA complex as an intermediate of in vitro genetic transcription.
    Proc Natl Acad Sci U S A. 1965 Dec;54(6):1736-43 PMID: 5218923
  47. Multiple forms of DNA-dependent RNA polymerase in eukaryotic organisms.
    Nature. 1969 Oct 18;224(5216):234-7 PMID: 5344598
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/6.2.507
Published
1979-02-00
Pages
507-23
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327710
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/6.2.507 PMC Full Text View journal details
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