The purification and properties of hen oviduct form B DNA-dependent RNA polymerase.

Nucleic acids research ·Vol. 1 ·No. 2 ·1974-02-00 ·Pages 299-308

Houghton M, Cox RF

Abstract

Hen oviduct form B DNA-dependent RNA polymerase has been extensively purified and its properties analysed. It seems likely to consist of a mixture of two forms of the type observed in tissues from other species. Furthermore using S1 nuclease to digest single-stranded DNA, we show that although form B can transcribe double-stranded DNA template it has a very strong preference for single-stranded regions. Also the rate of elongation on native DNA in vitro was measured and is an order of magnitude slower than that reported to be operative in vivo.

MeSH Terms

Animals Basidiomycota Centrifugation, Density Gradient Chickens Chromatography, DEAE-Cellulose Chromatography, Ion Exchange DNA DNA, Single-Stranded DNA-Directed RNA Polymerases/isolation & purification,metabolism Deoxyribonucleases Electrophoresis, Polyacrylamide Gel Female Kinetics Mycotoxins/pharmacology Oligopeptides/pharmacology Oviducts/enzymology Peptides, Cyclic/pharmacology Time Factors Transcription, Genetic Ultrasonics

Chemicals

DNA, Single-Stranded Mycotoxins Oligopeptides Peptides, Cyclic DNA DNA-Directed RNA Polymerases Deoxyribonucleases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Houghton M

Cox R F

References (24)

24 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/1.2.299

Published

1974-02-00

Pages

299-308

Language

English

Region

England

NLM ID

0411011

PMCID

PMC343348

Subset

External Links

PubMed source DOI: 10.1093/nar/1.2.299 PMC Full Text View journal details

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The purification and properties of hen oviduct form B DNA-dependent RNA polymerase.

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