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PMID: 3700603 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Method for the typing of Clostridium difficile based on polyacrylamide gel electrophoresis of [35S]methionine-labeled proteins.

Journal of clinical microbiology ·Vol. 23 ·No. 1 ·1986-01-00 ·Pages 197-8

Tabaqchali S, O'Farrell S, Holland D, Silman R

Abstract

A typing method for Clostridium difficile based on the incorporation of [35S]methionine into cellular proteins, their separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and their visualization by autoradiography is described. On analysis of the radiolabeled-protein profiles, nine distinct groups were observed (A to E and W to Z). The method, which is simple, reproducible, and readily expandable, has been applied in epidemiological studies to demonstrate cross-infection and hospital acquisition of C. difficile.

MeSH Terms
Adult Autoradiography Bacterial Proteins/analysis Clostridium/analysis,classification,isolation & purification Diarrhea/microbiology Electrophoresis, Polyacrylamide Gel Enterocolitis, Pseudomembranous/microbiology Feces/microbiology Humans Infant, Newborn Methionine Sulfur Radioisotopes
Chemicals
Bacterial Proteins Sulfur Radioisotopes Methionine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Tabaqchali S
O'Farrell S
Holland D
Silman R
References (17)
17 references, click to expand
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.23.1.197-198.1986
Published
1986-01-00
Pages
197-8
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC268603
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.23.1.197-198.1986 PMC Full Text View journal details
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