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PMID: 7161375 Published · ppublish English Journal Article

Investigation of an outbreak of antibiotic-associated colitis by various typing methods.

Journal of clinical microbiology ·Vol. 16 ·No. 6 ·1982-12-00 ·Pages 1096-101

Wüst J, Sullivan NM, Hardegger U, Wilkins TD

Abstract

During an outbreak of diarrheal disease due to Clostridium difficile in a surgical ward, 16 C. difficile isolates were cultured from fecal samples of 15 patients. Agarose gel electrophoresis for the detection of plasmid DNA, crossed immunoelectrophoresis for the detection of extracellular antigens and toxins, polyacrylamide gel electrophoresis for analyses of soluble proteins, assays for cytotoxicity, and a comparison of susceptibility to antimicrobial agents were employed. At least 12 of the 16 isolates were shown to be phenotypically the same strain. These findings suggest that in a hospital setting, diarrhea and pseudomembranous colitis caused by C. difficile can be of nosocomial origin and that they can spread from patient to patient.

MeSH Terms
Anti-Bacterial Agents/adverse effects Bacterial Proteins/isolation & purification Clostridium/isolation & purification Colitis/chemically induced,microbiology DNA, Bacterial/isolation & purification Diarrhea/microbiology Disease Outbreaks Humans Immunoelectrophoresis Microbial Sensitivity Tests Plasmids
Chemicals
Anti-Bacterial Agents Bacterial Proteins DNA, Bacterial
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Wüst J
Sullivan N M
Hardegger U
Wilkins T D
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35 references, click to expand
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.16.6.1096-1101.1982
Published
1982-12-00
Pages
1096-101
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC272546
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.16.6.1096-1101.1982 PMC Full Text View journal details
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