Home LiteratureArticle Details
PMID: 3693344 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Involvement of transferrin in the reduction of iron by the transplasma membrane electron transport system.

Journal of bioenergetics and biomembranes ·Vol. 19 ·No. 5 ·1987-10-00 ·Pages 535-49

Löw H, Grebing C, Lindgren A, Tally M, Sun IL, Crane FL

Abstract

Nonpermeable electron acceptors can be reduced by a transplasma membrane electron transport system in suspensions of intact cells. Here we report that diferric transferrin is reduced by HeLa S3 cells. The reduction is recorded spectrophotometrically as the formation of the ferrous complex of bathophenanthroline disulfonate. Ferric ammonium citrate can also be used as an electron acceptor and the presence of low concentrations of diferric transferrin greatly stimulates the reduction of trivalent iron under these conditions. Likewise very low concentrations of ferricyanide, which does not give rise to a ferrous bathophenanthroline disulfonate complex formation, have a strong stimulatory effect on the complex formation when ferric ammonium citrate is the source of ferric iron. Apotransferrin is a potent inhibitor of the reaction. The inhibition occurs at the concentration necessary for complete occupancy of the transferrin receptors. The inhibition can be demonstrated also when high concentrations of ferricyanide are used as electron acceptor. The possible mechanism behind the reported phenomena is discussed, and it is concluded that the transplasma membrane electron transport system can be involved in the process of cellular iron uptake.

MeSH Terms
Apoproteins/physiology Cell Membrane/metabolism Electron Transport Ferric Compounds/metabolism Ferricyanides/metabolism HeLa Cells Humans In Vitro Techniques Iron/metabolism Oxidation-Reduction Quaternary Ammonium Compounds/metabolism Receptors, Transferrin/metabolism Transferrin/metabolism,physiology
Chemicals
Apoproteins Ferric Compounds Ferricyanides Quaternary Ammonium Compounds Receptors, Transferrin Transferrin apotransferrin Iron ferric ammonium citrate
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Löw H
Department of Endocrinology, Karolinska Institute, Stockholm, Sweden.
Grebing C
Lindgren A
Tally M
Sun I L
Crane F L
References (41)
41 references, click to expand
  1. Competitive advantage of diferric transferrin in delivering iron to reticulocytes.
    Proc Natl Acad Sci U S A. 1983 Jan;80(1):300-4 PMID: 6572005
  2. The interaction of iron-conalbumin (anion) complexes with chick embryo red blood ccells.
    J Biol Chem. 1973 Aug 25;248(16):5848-53 PMID: 4723917
  3. Transferrin binding and iron uptake in mouse hepatocytes.
    Biochim Biophys Acta. 1983 Feb 16;762(1):102-10 PMID: 6830864
  4. Effects of anti-transferrin receptor antibodies on growth of normal and malignant myeloid cells.
    Int J Cancer. 1983 Sep 15;32(3):343-9 PMID: 6309680
  5. Equivalence of the two sites of human transferrin upon reduction with dithionite.
    Br J Haematol. 1977 Oct;37(2):302-3 PMID: 603763
  6. Thermodynamic binding constants of the zinc-human serum transferrin complex.
    Biochemistry. 1983 Aug 2;22(16):3920-6 PMID: 6615809
  7. Energetics of the calcium-transporting ATPase.
    J Biol Chem. 1984 Feb 10;259(3):1629-43 PMID: 6229538
  8. Effect of adenine nucleotides and pyrophosphate on the exchange of transferrin-bound carbonate.
    Biochim Biophys Acta. 1975 Dec 5;411(2):349-56 PMID: 172141
  9. Chelator-mediated iron efflux from reticulocytes.
    Biochim Biophys Acta. 1983 Aug 24;733(1):39-50 PMID: 6882755
  10. The interaction of transferrin with isolated hepatocytes.
    Biochim Biophys Acta. 1980 Dec 1;633(2):145-53 PMID: 7459386
  11. Reduction potential of iron in transferrin.
    Biochim Biophys Acta. 1985 Mar 8;838(3):295-301 PMID: 3970972
  12. Enzymatic iodination of polypeptides with 125I to high specific activity.
    Biochim Biophys Acta. 1971 Dec 28;251(3):363-9 PMID: 11452877
  13. Biological potency and binding affinity of monoiodoinsulin with iodine in tyrosine A14 or tyrosine A19.
    Biochem Biophys Res Commun. 1979 Apr 27;87(4):1183-90 PMID: 465032
  14. The effect of pH on the kinetics of iron release from human transferrin.
    Biochim Biophys Acta. 1982 Oct 28;719(1):140-6 PMID: 6816292
  15. Serum-free cell culture: a unifying approach.
    Cell. 1980 Dec;22(3):649-55 PMID: 7460009
  16. The transferrin cycle and iron uptake in rabbit reticulocytes. Pulse studies using 59Fe, 125I-labeled transferrin.
    J Biol Chem. 1983 Aug 25;258(16):9676-80 PMID: 6885764
  17. Iron transfer form transferrin to ferritin mediated by polyphosphate compounds.
    Biochim Biophys Acta. 1981 Nov 5;677(3-4):417-23 PMID: 6271255
  18. Membrane proteins synthesized by rabbit reticulocytes.
    J Cell Biol. 1975 Apr;65(1):51-64 PMID: 1127015
  19. Transferrin receptor: its biological significance.
    J Membr Biol. 1985;88(3):205-15 PMID: 3007762
  20. A transmembranous NADH-dehydrogenase in human erythrocyte membranes.
    J Bioenerg Biomembr. 1984 Dec;16(5-6):517-33 PMID: 6537435
  21. Is divalent iron bound to transferrin?
    Biochim Biophys Acta. 1970 Nov 17;221(2):228-33 PMID: 5490230
  22. Factors affecting the adenosine triphosphate induced release of iron from transferrin.
    Biochemistry. 1978 Jan 10;17(1):167-72 PMID: 22345
  23. Transferrin-bipyridine iron transfer mediated by haemoproteins.
    Biochim Biophys Acta. 1980 May 7;629(2):391-8 PMID: 6446325
  24. Transferrin recycling in reticulocytes: pH and iron are important determinants of ligand binding and processing.
    Biochem Biophys Res Commun. 1983 Jun 15;113(2):650-8 PMID: 6870878
  25. Autoradiographic localization of 125-I-labelled transferrin in rabbit reticulocytes.
    Nature. 1969 Sep 27;223(5213):1371-2 PMID: 5809513
  26. Properties of a transplasma membrane electron transport system in HeLa cells.
    J Bioenerg Biomembr. 1984 Dec;16(5-6):583-95 PMID: 6537437
  27. The role of the anion-binding site of transferrin in its interaction with the reticulocyte.
    Biochim Biophys Acta. 1973 May 28;304(3):797-804 PMID: 4353699
  28. Receptor-mediated endocytosis of transferrin in developmentally totipotent mouse teratocarcinoma stem cells.
    J Biol Chem. 1981 Apr 10;256(7):3245-52 PMID: 6259157
  29. Transplasma membrane redox stimulates HeLa cell growth.
    Biochem Biophys Res Commun. 1984 Dec 14;125(2):649-54 PMID: 6393980
  30. Uptake of iron from transferrin by isolated hepatocytes.
    Biochim Biophys Acta. 1984 Jun 19;804(2):200-8 PMID: 6326850
  31. The significance of transferrin-bound bicarbonate in the uptake of iron by reticulocytes.
    Biochim Biophys Acta. 1973 May 28;304(3):805-13 PMID: 4726859
  32. Evidence for the extracellular reduction of ferricyanide by rat liver. A trans-plasma membrane redox system.
    Biochem J. 1981 Dec 15;200(3):565-72 PMID: 6282252
  33. The reduction and release of iron from Fe3+ .transferrin.CO3(2-).
    J Biol Chem. 1979 Sep 25;254(18):8847-54 PMID: 479164
  34. Transfer of iron from serum iron-binding protein to human reticulocytes.
    J Clin Invest. 1959 Jan 1;38(1, Part 1):161-85 PMID: 13620780
  35. Distribution of receptors for transferrin and low density lipoprotein on the surface of giant HeLa cells.
    Proc Natl Acad Sci U S A. 1983 Jan;80(2):454-8 PMID: 6300844
  36. Iron transport and storage proteins.
    Annu Rev Biochem. 1980;49:357-93 PMID: 6996567
  37. Heme regulation of HeLa cell transferrin receptor number.
    J Biol Chem. 1984 Nov 10;259(21):13235-40 PMID: 6092369
  38. The reticulocyte plasma membrane pathway of iron uptake as determined by the mechanism of alpha, alpha'-dipyridyl inhibition.
    J Biol Chem. 1983 Jan 25;258(2):1146-51 PMID: 6401715
  39. The preparation, properties, and metabolism of 14C-bicarbonate-labelled transferrin.
    Biochem Biophys Res Commun. 1973 Jul 2;53(1):32-8 PMID: 4741552
  40. Effect of pH and iron content of transferrin on its binding to reticulocyte receptors.
    Biochim Biophys Acta. 1983 Jul 14;762(4):498-502 PMID: 6307387
  41. Transferrin receptor induction in mitogen-stimulated human T lymphocytes is required for DNA synthesis and cell division and is regulated by interleukin 2.
    Proc Natl Acad Sci U S A. 1983 Jun;80(11):3494-8 PMID: 6304712
Article Info
Journal
Journal of bioenergetics and biomembranes
Abbr.
J Bioenerg Biomembr
ISSN
0145-479X
DOI
10.1007/BF00770036
Published
1987-10-00
Pages
535-49
Language
English
Region
United States
NLM ID
7701859
Subset
IM
Grants
PHS HHS · K6 29831 · United States
External Links
PubMed source DOI: 10.1007/BF00770036 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2