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PMID: 1127015 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Membrane proteins synthesized by rabbit reticulocytes.

The Journal of cell biology ·Vol. 65 ·No. 1 ·1975-04-00 ·Pages 51-64

Lodish HF, Small B

Abstract

Intact rabbit reticulocyte cells synthesize two predominant species of polypeptides which are components of the cell plasma membrane. Previous work (Lodish, H. F. 1973. Proc. Natl. Acad. Sci. U. S. A. 70:1526-1530.) showed that these proteins were synthesized by polyribosomes not attached to membranes. We show here that both polypeptides are confined to the cytoplasmic surface of the cell membrane. These studies utilized iodination of whole cells and of membranes with lactoperoxidase, and digestion of whole cells and membranes with chymotrypsin, One of these proteins is synthesized as a precursor, and about 20-40 amino acids are removed after it is incorporated into the membrane, We discuss the probable sites of synthesis of these and other classes of membrane proteins.

MeSH Terms
Animals Blood Proteins/biosynthesis Cell Membrane/metabolism Centrifugation, Density Gradient Chymotrypsin Electrophoresis, Polyacrylamide Gel Iodine Radioisotopes Peroxidases Polyribosomes/metabolism Rabbits Reticulocytes/metabolism
Chemicals
Blood Proteins Iodine Radioisotopes Peroxidases Chymotrypsin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lodish H F
Small B
References (34)
34 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.65.1.51
Published
1975-04-00
Pages
51-64
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2111166
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.65.1.51 PMC Full Text View journal details
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