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PMID: 3682059 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The duck hepatitis B virus pre-C region encodes a signal sequence which is essential for synthesis and secretion of processed core proteins but not for virus formation.

Journal of virology ·Vol. 61 ·No. 12 ·1987-12-00 ·Pages 3701-9

Schlicht HJ, Salfeld J, Schaller H

Abstract

Analysis of the serum of duck hepatitis B virus (DHBV)-infected ducks has revealed the presence of C-terminally truncated viral core proteins (e antigens). These proteins are glycosylated and therefore were not released from infected cells by lysis but rather by active secretion, indicating that the DHBV core protein can be synthesized alternatively as a cytoplasmic or a secretory protein. Transient expression of cloned wild-type DHBV DNA and of a specifically designed viral mutant in a human hepatoma cell line (Hep-G2) showed that the DHBV core gene promoter is active in differentiated human liver cells and that synthesis and secretion of the processed core proteins are dependent on the expression of the pre-C region, a small open reading frame which precedes the core gene. In addition, these experiments showed that the mechanism of core protein processing and secretion is conserved between DHBV and the human hepatitis B virus and therefore might be important for the hepatitis B virus life cycle in general. In spite of this, intrahepatic injection of the pre-C mutant into uninfected ducks resulted in viremia without concomitant e-antigen synthesis, indicating that virus formation is independent of pre-C expression.

MeSH Terms
Animals Antigens, Viral/biosynthesis,genetics,metabolism Cell Line Ducks Gene Expression Regulation Genes, Viral Glycosylation Hepatitis B/microbiology Hepatitis B virus/genetics,metabolism,physiology Humans Immunoassay Mutation Promoter Regions, Genetic Protein Sorting Signals/genetics Transfection Viral Core Proteins/biosynthesis,genetics,metabolism Viremia/microbiology Virus Replication
Chemicals
Antigens, Viral Protein Sorting Signals Viral Core Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Schlicht H J
Zentrum für Molekulare Biologie, University of Heidelberg, Federal Republic of Germany.
Salfeld J
Schaller H
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.61.12.3701-3709.1987
Published
1987-12-00
Pages
3701-9
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC255982
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.61.12.3701-3709.1987 PMC Full Text View journal details
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