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PMID: 3681953 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Collagen receptors mediate early events in the attachment of epithelial (MDCK) cells.

The Journal of membrane biology ·Vol. 98 ·No. 3 ·1987-00-00 ·Pages 223-36

Salas PJ, Vega-Salas DE, Rodriguez-Boulan E

Abstract

Madin-Darby canine kidney (MDCK) cells kept in suspension culture for 12-15 hr displayed high-affinity binding sites for 125I-lathyritic (soluble) collagen (120,000/cell, KD = 30 nM) and preferred collagens types I and IV over laminin or fibronectin as substrates during the first hour of attachment. On the other hand, after 4 hr, attachment to all four substrates was equally efficient. Upon challenge with a collagen substrate, the high-affinity sites were rapidly recruited on it (T1/2 = 6 min). Their occupancy by soluble collagen triggered the exocytosis of a second large population of low-affinity collagen binding sites that included laminin and seems to be involved in a second cell-attachment mechanism. These results are compatible with a two-step model of MDCK cell attachment to the substrate: first, via high-affinity collagen binding sites, and second, via laminin of cellular origin.

MeSH Terms
Animals Carrier Proteins/physiology Cell Adhesion Cell Line Collagen Epithelial Cells Fibronectins Kinetics Laminin Receptors, Collagen Receptors, Immunologic/physiology
Chemicals
Carrier Proteins Fibronectins Laminin Receptors, Collagen Receptors, Immunologic Collagen
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Salas P J
Department of Cell Biology and Anatomy, Cornell University Medical College, New York, New York 10021.
Vega-Salas D E
Rodriguez-Boulan E
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Article Info
Journal
The Journal of membrane biology
Abbr.
J Membr Biol
ISSN
0022-2631
DOI
10.1007/BF01871185
Published
1987-00-00
Pages
223-36
Language
English
Region
United States
NLM ID
0211301
Subset
IM
Grants
NIADDK NIH HHS · AM 26481 · United States
NIGMS NIH HHS · GM 34107 · United States
External Links
PubMed source DOI: 10.1007/BF01871185 View journal details
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