Home LiteratureArticle Details
PMID: 3997977 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Binding of laminin to type IV collagen: a morphological study.

The Journal of cell biology ·Vol. 100 ·No. 6 ·1985-06-00 ·Pages 1848-53

Charonis AS, Tsilibary EC, Yurchenco PD, Furthmayr H

Abstract

A mixture of laminin and type IV collagen was analyzed by rotary shadowing using carbon/platinum and electron microscopy. Laminin was found to form distinct complexes with type IV collagen: one site of interaction is located 140 nm from the COOH-terminal, noncollagenous (NC1) domain and the other is located within the NH2-terminal region. The isolated NC1 fragment of type IV collagen does not appear to interact with laminin, while pepsin-treated type IV collagen, which lacks the NC1 domain, retains its ability to form complexes with laminin. Analysis of the laminin-type IV complexes indicates that laminin binds to type IV collagen via the globular regions of either of its four arms. This finding is supported by experiments using fragment P1 of laminin which lacks the globular regions and which does not bind to type IV collagen in a specific way. In addition, after heat-denaturation of laminin no specific binding is observed.

MeSH Terms
Animals Binding Sites Carbon Collagen/metabolism Hot Temperature Laminin/metabolism Macromolecular Substances Mice Microscopy, Electron Peptide Fragments/metabolism Platinum Protein Binding Protein Denaturation
Chemicals
Laminin Macromolecular Substances Peptide Fragments Platinum Carbon Collagen
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Charonis A S
Tsilibary E C
Yurchenco P D
Furthmayr H
References (21)
21 references, click to expand
  1. Nature of the collagenous protein in a tumor basement membrane.
    Eur J Biochem. 1978 Mar;84(1):43-52 PMID: 648517
  2. Cell adhesive, protein binding, and antigenic properties of laminin.
    Coll Relat Res. 1983 Sep;3(5):359-69 PMID: 6641122
  3. Human laminin isolated in a nearly intact, biologically active form from placenta by limited proteolysis.
    J Biol Chem. 1983 Oct 25;258(20):12654-60 PMID: 6415055
  4. The molecular structure of human erythrocyte spectrin. Biophysical and electron microscopic studies.
    J Mol Biol. 1979 Jun 25;131(2):303-29 PMID: 490648
  5. Discontinuities in the triple helical sequence Gly-X-Y of basement membrane (type IV) collagen.
    FEBS Lett. 1980 Jun 30;115(2):297-300 PMID: 6772473
  6. Properties of a basement membrane-related glycoprotein synthesized in culture by a mouse embryonal carcinoma-derived cell line.
    Cell. 1979 Feb;16(2):277-87 PMID: 88263
  7. Comparative distribution of laminin, type IV collagen, and fibronectin in the rat glomerulus.
    J Histochem Cytochem. 1982 Sep;30(9):874-86 PMID: 7130672
  8. Isolation of a laminin-binding protein from muscle cell membranes.
    EMBO J. 1983;2(6):861-5 PMID: 16453457
  9. Self-assembly of basement membrane collagen.
    Biochemistry. 1984 Apr 10;23(8):1839-50 PMID: 6722126
  10. Laminin--a glycoprotein from basement membranes.
    J Biol Chem. 1979 Oct 10;254(19):9933-7 PMID: 114518
  11. Laminin receptor on human breast carcinoma cells.
    Proc Natl Acad Sci U S A. 1983 Jan;80(2):444-8 PMID: 6300843
  12. Methods in laboratory investigation. Monoclonal antibodies to type IV collagen: probes for the study of structure and function of basement membranes.
    Lab Invest. 1983 May;48(5):639-49 PMID: 6682465
  13. A network model for the organization of type IV collagen molecules in basement membranes.
    Eur J Biochem. 1981 Nov;120(2):203-11 PMID: 6274634
  14. Formation of a supramolecular complex is involved in the reconstitution of basement membrane components.
    Biochemistry. 1983 Oct 11;22(21):4969-74 PMID: 6227336
  15. Localization of type IV collagen, laminin, heparan sulfate proteoglycan, and fibronectin to the basal lamina of basement membranes.
    J Cell Biol. 1982 Oct;95(1):340-4 PMID: 6216257
  16. A murine tumor producing a matrix of basement membrane.
    J Exp Med. 1977 Jan 1;145(1):204-20 PMID: 830788
  17. Isolation and characterization of type IV procollagen, laminin, and heparan sulfate proteoglycan from the EHS sarcoma.
    Biochemistry. 1982 Nov 23;21(24):6188-93 PMID: 6217835
  18. Shapes, domain organizations and flexibility of laminin and fibronectin, two multifunctional proteins of the extracellular matrix.
    J Mol Biol. 1981 Jul 25;150(1):97-120 PMID: 6795355
  19. Characterization of pepsin fragments of laminin in a tumor basement membrane. Evidence for the existence of related proteins.
    Hoppe Seylers Z Physiol Chem. 1980 Nov;361(11):1651-60 PMID: 6778807
  20. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  21. Structure and interactions of heparan sulfate proteoglycans from a mouse tumor basement membrane.
    Eur J Biochem. 1984 Aug 15;143(1):145-57 PMID: 6236080
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.100.6.1848
Published
1985-06-00
Pages
1848-53
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2113590
Subset
IM
Grants
NIADDK NIH HHS · AM-30556 · United States
NIGMS NIH HHS · GM-07562 · United States
External Links
PubMed source DOI: 10.1083/jcb.100.6.1848 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2