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PMID: 3670314 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

One- and two-step transformations of rat thyroid epithelial cells by retroviral oncogenes.

Molecular and cellular biology ·Vol. 7 ·No. 9 ·1987-09-00 ·Pages 3365-70

Fusco A, Berlingieri MT, Di Fiore PP, Portella G, Grieco M, Vecchio G

Abstract

A system of epithelial cells is described in which it is possible to study the number and the nature of genes capable of conferring the malignant phenotype. Two fully differentiated, hormone-responsive cell lines from rat thyroid glands are presented which are susceptible to one-step or two-step transformation upon infection with several murine acute retroviruses. After infection, both cell lines became independent from their thyrotropic hormone requirement for growth. However, complete transformation was achieved with one of the cell lines (FRTL-5 Cl 2), whereas the other cell line (PC Cl 3) failed to grow in agar and to give rise to tumors in vivo. The latter cell line was susceptible to complete transformation upon cooperation of the v-ras-Ha and the human c-myc oncogenes.

MeSH Terms
Animals Cell Differentiation Cell Line, Transformed Cell Transformation, Viral Epithelial Cells Epithelium/physiology Iodides/metabolism Neoplasms, Experimental/genetics Oncogenes RNA, Messenger/genetics RNA, Neoplasm/genetics Rats Retroviridae/genetics,pathogenicity Thyroglobulin/biosynthesis Thyroid Gland/cytology,physiology
Chemicals
Iodides RNA, Messenger RNA, Neoplasm Thyroglobulin
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Fusco A
Dipartimento di Biologia e Patologia Cellulare e Molecolare, L. Califano II Facoltà di Medicina e Chirurgia, Università di Napoli, Italy.
Berlingieri M T
Di Fiore P P
Portella G
Grieco M
Vecchio G
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.7.9.3365-3370.1987
Published
1987-09-00
Pages
3365-70
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC367981
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.7.9.3365-3370.1987 PMC Full Text View journal details
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