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PMID: 361653 Published · ppublish English Comparative Study Journal Article

The size of the fibre populations in rabbit skeletal muscles as revealed by indirect immunofluorescence with anti-myosin sera.

Histochemistry ·Vol. 57 ·No. 3 ·1978-09-15 ·Pages 223-35

Lutz H, Ermini M, Jenny E

Abstract

Antibodies against myosin of the fast long. Dorsi and the slow soleus muscle of rabbits were induced in guinea pigs. With the aid of a new technique, the gel-electrophoresis-derived-enzyme-linked-immunosorbent assay (GEDELISA) it could be shown that they are directed against the heavy and the light chains of fast (M. long. dorsi) and slow (M. soleus) myosin. In the indirect immunofluorescence test each antiserum only stained one population of fibres in five different muscles tested. The single fibres were observed to react only with one of the two types of antisera. The following percentage of fibres showed a positive reaction with the anti-fast myosin serum: M. long. dorsi, 95%; M. psoa maior, 95%, M. psoa minor, 92%; M. tibialis ant., 90%; M. soleus, 15%.

MeSH Terms
Animals Enzyme-Linked Immunosorbent Assay/methods Fluorescent Antibody Technique Muscles/cytology Myosins/immunology Rabbits/anatomy & histology
Chemicals
Myosins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lutz H
Ermini M
Jenny E
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33 references, click to expand
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Article Info
Journal
Histochemistry
Abbr.
Histochemistry
ISSN
0301-5564
DOI
10.1007/BF00492082
Published
1978-09-15
Pages
223-35
Language
English
Region
Germany
NLM ID
0411300
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00492082
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