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PMID: 3612087 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Calcium- and voltage-activated plateau currents of cardiac Purkinje fibers.

The Journal of general physiology ·Vol. 89 ·No. 6 ·1987-06-00 ·Pages 921-58

Kenyon JL, Sutko JL

Abstract

We have used the two-microelectrode voltage-clamp technique to investigate the components of membrane current that contribute to the formation of the early part of the plateau phase of the action potential of calf cardiac Purkinje fibers. 3,4-Diaminopyridine (50 microM) reduced the net transient outward current elicited by depolarizations to potentials positive to -30 mV but had no consistent effect on contraction. We attribute this effect to the blockade of a voltage-activated transient potassium current component. Ryanodine (1 microM), an inhibitor of sarcoplasmic reticulum calcium release and intracellular calcium oscillations in Purkinje fibers (Sutko, J.L., and J.L. Kenyon. 1983. Journal of General Physiology. 82:385-404), had complex effects on membrane currents as it abolished phasic contractions. At early times during a depolarization (5-30 ms), ryanodine reduced the net outward current. We attribute this effect to the loss of a component of calcium-activated potassium current caused by the inhibition of sarcoplasmic reticulum calcium release and the intracellular calcium transient. At later times during a depolarization (50-200 ms), ryanodine increased the net outward current. This effect was not seen in low-sodium solutions and we could not observe a reversal potential over a voltage range of -100 to +75 mV. These data suggest that the effect of ryanodine on the late membrane current is attributable to the loss of sodium-calcium exchange current caused by the inhibition of sarcoplasmic reticulum calcium release and the intracellular calcium transient. Neither effect of ryanodine was dependent on chloride ions, which suggests that chloride ions do not carry the ryanodine-sensitive current components. Strontium (2.7 mM replacing calcium) and caffeine (10 mM), two other treatments that interfere with sarcoplasmic reticulum function, had effects in common with ryanodine. This supports the hypothesis that the effects of ryanodine may be attributed to the inhibition of sarcoplasmic reticulum calcium release.

MeSH Terms
Action Potentials Aminopyridines/pharmacology Animals Calcium/metabolism Cattle Cell Membrane/drug effects,physiology Chlorides/metabolism Electric Conductivity Heart Conduction System/physiology Microelectrodes Purkinje Fibers/drug effects,physiology Ryanodine/pharmacology Sarcoplasmic Reticulum/metabolism
Chemicals
Aminopyridines Chlorides Ryanodine Calcium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kenyon J L
Sutko J L
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Article Info
Journal
The Journal of general physiology
Abbr.
J Gen Physiol
ISSN
0022-1295
DOI
10.1085/jgp.89.6.921
Published
1987-06-00
Pages
921-58
Language
English
Region
United States
NLM ID
2985110R
PMCID
PMC2215964
Subset
IM
Grants
NHLBI NIH HHS · HL-17669 · United States
NHLBI NIH HHS · HL-26528 · United States
External Links
PubMed source DOI: 10.1085/jgp.89.6.921 PMC Full Text View journal details
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