Photoelectric recording of mechanical responses of cardiac myocytes.

Pflugers Archiv : European journal of physiology ·Vol. 408 ·No. 4 ·1987-04-00 ·Pages 390-4

Meyer R, Wiemer J, Dembski J, Haas HG

Abstract

A method to monitor contraction of isolated myocytes by transmicroscopic photometry is illustrated. Two photodiodes are mounted inside an inverse microscope used for visual control of a cell. Illumination of one diode varies in proportion to changes in cell length. The contraction signal is amplified in a comparator circuit. Spatial resolution of the device is in the order of 1 micron which corresponds to about 5% of cell shortening in the fully activated state of contraction. The method was tested on isolated myocytes from guinea-pig ventricle. Optical records of contraction in response to action potentials or during voltage clamp compare well with the contractile behavior of multicellular preparations.

MeSH Terms

Action Potentials Animals Calcium/physiology Guinea Pigs Heart/physiology Myocardial Contraction Myocardium/cytology

Chemicals

Calcium

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Meyer R

Wiemer J

Dembski J

Haas H G

References (16)

16 references, click to expand

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Article Info

Journal

Pflugers Archiv : European journal of physiology

Abbr.

Pflugers Arch

ISSN

0031-6768

DOI

10.1007/BF00581134

Published

1987-04-00

Pages

390-4

Language

English

Region

Germany

NLM ID

0154720

Subset

External Links

PubMed source DOI: 10.1007/BF00581134 View journal details

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Photoelectric recording of mechanical responses of cardiac myocytes.

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