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PMID: 3573155 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Splice acceptor site for the env message of Moloney murine leukemia virus.

Journal of virology ·Vol. 61 ·No. 6 ·1987-06-00 ·Pages 2038-41

Lazo PA, Prasad V, Tsichlis PN

Abstract

We report the isolation and sequence of a cDNA clone containing part of the env message of the Moloney murine leukemia virus (MoMuLV). This clone was derived from a rat thymic lymphoma induced by MoMuLV. The AG acceptor site employed in this message is located at position 5490 in the MoMuLV genome. This splice site is detectable at the cDNA level by the creation of a novel SacI restriction site not present in the viral genome. In the -1 to -40 region, this AG acceptor site is preceded by four conserved heptanucleotides (PyXPyTPuAPy) that may function as acceptors for removal of the 5' end of the intron.

MeSH Terms
Base Sequence DNA/genetics DNA, Viral/genetics Genes Genes, Viral Moloney murine leukemia virus/genetics RNA Splicing RNA, Messenger/genetics,metabolism RNA, Viral/genetics,metabolism Viral Envelope Proteins/genetics
Chemicals
DNA, Viral RNA, Messenger RNA, Viral Viral Envelope Proteins DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lazo P A
Prasad V
Tsichlis P N
References (26)
26 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.61.6.2038-2041.1987
Published
1987-06-00
Pages
2038-41
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC254215
Subset
IM
Grants
NCI NIH HHS · CA06927 · United States
NCI NIH HHS · CA38047 · United States
NCRR NIH HHS · RR05539 · United States
Databases
GENBANK
M16686, M16687
External Links
PubMed source DOI: 10.1128/JVI.61.6.2038-2041.1987 PMC Full Text View journal details
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