Home LiteratureArticle Details
PMID: 3573154 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Comparison of the relative roles of the F and HN surface glycoproteins of the paramyxovirus simian virus 5 in inducing protective immunity.

Journal of virology ·Vol. 61 ·No. 6 ·1987-06-00 ·Pages 1972-7

Paterson RG, Lamb RA, Moss B, Murphy BR

Abstract

To compare the relative roles of the paramyxovirus simian virus 5 (SV5) major surface glycoproteins, fusion (F) and hemagglutinin-neuraminidase (HN), in inducing protective immunity, two recombinant vaccinia viruses were constructed. The F and HN polypeptides expressed by the recombinant viruses were indistinguishable from their authentic SV5 counterparts in electrophoretic mobility, glycosylation, and, for the F protein, cleavage of the precursor, F0, to the disulfide-linked subunits F1 and F2. Injection of rabbits and hamsters with live recombinant virus elicited an antibody response to either F or HN and provided a source of monospecific polyclonal antisera to the SV5 proteins. The vaccinia virus-SV5 F (vaccinia-F) recombinant induced higher levels of neutralizing antibody than did the vaccinia-HN recombinant, but animals inoculated with vaccinia-HN were better protected from challenge with SV5. Animals infected with both the vaccinia-HN and vaccinia-F viruses were nearly as well protected from challenge as were animals infected with SV5.

MeSH Terms
Animals Antibodies, Viral/biosynthesis Antigens/immunology Antigens, Viral/genetics,immunology Cricetinae Female HN Protein Immunization Mesocricetus Paramyxoviridae/genetics,immunology Recombinant Proteins/genetics,immunology Vaccines, Synthetic/immunology Vaccinia virus/genetics Viral Envelope Proteins/genetics,immunology Viral Fusion Proteins/genetics,immunology Viral Vaccines/immunology
Chemicals
Antibodies, Viral Antigens Antigens, Viral HN Protein Recombinant Proteins Vaccines, Synthetic Viral Envelope Proteins Viral Fusion Proteins Viral Vaccines
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Paterson R G
Lamb R A
Moss B
Murphy B R
References (29)
29 references, click to expand
  1. Isolation of paramyxovirus glycoproteins. Association of both hemagglutinating and neuraminidase activities with the larger SV5 glycoprotein.
    Virology. 1972 Dec;50(3):640-52 PMID: 4118317
  2. EFFECT OF ANTISERUM ON THE CELL FUSION REACTION CAUSED BY HVJ.
    Virology. 1964 Mar;22:397-409 PMID: 14127837
  3. Effect of specific antibodies on biological functions of the envelope components of Newcastle disease virus.
    Virology. 1974 Oct;61(2):354-60 PMID: 4213724
  4. The hemagglutinating and neuraminidase protein of a paramyxovirus: interaction with neuraminic acid in affinity chromatography.
    Virology. 1974 Nov;62(1):125-33 PMID: 4138483
  5. Identification of measles virus-specific hemolysis-inihibiting antibodies separate from hemagglutination-inhibiting antibodies.
    Infect Immun. 1975 Feb;11(2):231-9 PMID: 803467
  6. Differences in the appearance of antibodies to structural components of measles virus after immunization with inactivated and live virus.
    J Infect Dis. 1975 Sep;132(3):262-9 PMID: 1159329
  7. Polypeptide synthesis in simian virus 5-infected cells.
    J Virol. 1977 Jul;23(1):177-87 PMID: 196101
  8. Immunologic properties of purified Sendai virus glycoproteins.
    J Immunol. 1977 Dec;119(6):1882-7 PMID: 72108
  9. Evidence for a ninth influenza viral polypeptide.
    Virology. 1978 Nov;91(1):60-78 PMID: 726267
  10. Reconstitution of membranes with individual paramyxovirus glycoproteins and phospholipid in cholate solution.
    Virology. 1979 Jun;95(2):476-91 PMID: 223289
  11. Importance of antibodies to the fusion glycoprotein of paramyxoviruses in the prevention of spread of infection.
    J Exp Med. 1980 Feb 1;151(2):275-88 PMID: 6766174
  12. Separation of Sendai virus glycoproteins by using glutaraldehyde-treated erythrocytes and preparation of monospecific antisera against the glycoproteins.
    Infect Immun. 1980 Oct;30(1):212-8 PMID: 6254880
  13. Immunological studies of the functions of paramyxovirus glycoproteins.
    Virology. 1981 Feb;109(1):94-105 PMID: 7467135
  14. Hemagglutinin-specific enzyme-linked immunosorbent assay for antibodies to influenza A and B viruses.
    J Clin Microbiol. 1981 Mar;13(3):554-60 PMID: 7240388
  15. Mapping of the vaccinia virus thymidine kinase gene by marker rescue and by cell-free translation of selected mRNA.
    Proc Natl Acad Sci U S A. 1982 Feb;79(4):1210-4 PMID: 6280173
  16. Vaccinia virus: a selectable eukaryotic cloning and expression vector.
    Proc Natl Acad Sci U S A. 1982 Dec;79(23):7415-9 PMID: 6296831
  17. Antigenic characterization of influenza A virus matrix protein with monoclonal antibodies.
    J Virol. 1984 Jan;49(1):248-52 PMID: 6197538
  18. Purification, morphology and antigenic characterization of measles virus envelope components.
    J Gen Virol. 1984 Feb;65 ( Pt 2):355-66 PMID: 6198452
  19. Vaccinia virus recombinants: expression of VSV genes and protective immunization of mice and cattle.
    Science. 1985 Jan 25;227(4685):433-5 PMID: 2981435
  20. Eukaryotic transient expression system dependent on transcription factors and regulatory DNA sequences of vaccinia virus.
    Proc Natl Acad Sci U S A. 1985 Jan;82(1):19-23 PMID: 3855541
  21. Hemagglutinin-neuraminidase protein of the paramyxovirus simian virus 5: nucleotide sequence of the mRNA predicts an N-terminal membrane anchor.
    J Virol. 1985 Apr;54(1):1-6 PMID: 3973974
  22. Expression at the cell surface of biologically active fusion and hemagglutinin/neuraminidase proteins of the paramyxovirus simian virus 5 from cloned cDNA.
    Proc Natl Acad Sci U S A. 1985 Nov;82(22):7520-4 PMID: 3865176
  23. Monoclonal antibodies to hemagglutinin-neuraminidase and fusion glycoproteins of Newcastle disease virus: relationship between glycosylation and reactivity.
    J Virol. 1986 Mar;57(3):1198-202 PMID: 2419585
  24. Enhancement of respiratory syncytial virus pulmonary pathology in cotton rats by prior intramuscular inoculation of formalin-inactiva ted virus.
    J Virol. 1986 Mar;57(3):721-8 PMID: 2419587
  25. Protection against canine distemper virus in dogs after immunization with isolated fusion protein.
    J Virol. 1986 May;58(2):536-41 PMID: 3754590
  26. Expression of the F glycoprotein of respiratory syncytial virus by a recombinant vaccinia virus: comparison of the individual contributions of the F and G glycoproteins to host immunity.
    Proc Natl Acad Sci U S A. 1986 Oct;83(19):7462-6 PMID: 3532115
  27. The purification fo four strains of poxvirus.
    Virology. 1962 Sep;18:9-18 PMID: 14036977
  28. MULTIPLICATION OF A MYXOVIRUS (SV5) WITH MINIMAL CYTOPATHIC EFFECTS AND WITHOUT INTERFERENCE.
    Virology. 1964 Jun;23:224-33 PMID: 14192298
  29. Identification of biological activities of paramyxovirus glycoproteins. Activation of cell fusion, hemolysis, and infectivity of proteolytic cleavage of an inactive precursor protein of Sendai virus.
    Virology. 1974 Feb;57(2):475-90 PMID: 4361457
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.61.6.1972-1977.1987
Published
1987-06-00
Pages
1972-7
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC254205
Subset
IM
Grants
NIAID NIH HHS · AI-20201 · United States
NIAID NIH HHS · AI-23173 · United States
External Links
PubMed source DOI: 10.1128/JVI.61.6.1972-1977.1987 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2