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PMID: 3562229 Published · ppublish English Journal Article

A new moderately repetitive DNA sequence family of novel organization.

Nucleic acids research ·Vol. 15 ·No. 5 ·1987-03-11 ·Pages 2327-41

Epstein ND, Karlsson S, O'Brien S, Modi W, Moulton A, Nienhuis AW

Abstract

In cloning adenovirus homologous sequences, from a human cosmid library, we identified a moderately repetitive DNA sequence family consisting of tandem arrays of 2.5 kb members. A member was sequenced and several non-adjacent, 15-20 bp G-C rich segments with homology to the left side of adenovirus were discovered. The copy number of 400 members is highly conserved among humans. Southern blots of partial digests of human DNA have verified the tandem array of the sequence family. The chromosomal location was defined by somatic cell genetics and in situ hybridization. Tandem arrays are found only on chromosomes 4 (4q31) and 19 (q13.1-q13.5). Homologous repetitive sequences are found in DNA of other primates but not in cat or mouse. Thus we have identified a new family of moderately repetitive DNA sequences, unique because of its organization in clustered tandem arrays, its length, its chromosomal location, and its lack of homology to other moderately repetitive sequence families.

MeSH Terms
Adenoviruses, Human/genetics Base Sequence Cloning, Molecular Cosmids DNA/genetics Genes, Viral Humans Repetitive Sequences, Nucleic Acid Sequence Homology, Nucleic Acid
Chemicals
DNA
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Epstein N D
Karlsson S
O'Brien S
Modi W
Moulton A
Nienhuis A W
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50 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.5.2327
Published
1987-03-11
Pages
2327-41
Language
English
Region
England
NLM ID
0411011
PMCID
PMC340637
Subset
IM
Databases
GENBANK
X04912
External Links
PubMed source DOI: 10.1093/nar/15.5.2327 PMC Full Text View journal details
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