Home LiteratureArticle Details
PMID: 355225 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Regulation of the synthesis of ribonucleoside diphosphate reductase in Escherichia coli: specific activity of the enzyme in relationship to perturbations of DNA replication.

Journal of bacteriology ·Vol. 135 ·No. 2 ·1978-08-00 ·Pages 429-35

Filpula D, Fuchs JA

Abstract

Ribonucleoside diphosphate reductase (RDP reductase) activity was found to greatly increase after a shift to the nonpermissive temperature in Escherichia coli mutants temperature sensitive for DNA elongation (dnaE dnaG dnaZ lig) or DNA initiation (dnaA dnaC dnaI). However, the kinetics of increase in RDP reductase after a shift to nonpermissive conditions were significantly different in initiation-defective mutants compared with elongation-defective mutants. In strains without defects in DNA metabolism, the specific activity of RDP reductase was found to increase with increasing growth rate. Nutritional shifts to faster growth conditions caused cells to transiently overproduce RDP reductase before adjusting to the new steady-state conditions.

MeSH Terms
DNA Replication DNA, Bacterial/biosynthesis Escherichia coli/enzymology,genetics,growth & development Genes Mutation Ribonucleoside Diphosphate Reductase/biosynthesis Ribonucleotide Reductases/biosynthesis Temperature
Chemicals
DNA, Bacterial Ribonucleotide Reductases Ribonucleoside Diphosphate Reductase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Filpula D
Fuchs J A
References (28)
28 references, click to expand
  1. Mutants of Escherichia coli requiring methionine or vitamin B12.
    J Bacteriol. 1950 Jul;60(1):17-28 PMID: 15436457
  2. MECHANISM OF ACTION OF NALIDIXIC ACID ON ESCHERICHIA COLI.II. INHIBITION OF DEOXYRIBONUCLEIC ACID SYNTHESIS.
    J Bacteriol. 1965 Apr;89:1068-74 PMID: 14276097
  3. INDUCTION OF REPLICATION BY THYMINE STARVATION AT THE CHROMOSOME ORIGIN IN ESCHERICHIA COLI.
    J Mol Biol. 1964 Aug;9:288-307 PMID: 14202267
  4. THE EFFECT OF AMINO ACID DEPRIVATION ON SUBSEQUENT DEOXYRIBONUCLEIC ACID REPLICATION.
    Biochim Biophys Acta. 1963 Sep 17;76:9-24 PMID: 14068565
  5. Thymine deficiency and the normal DNA replication cycle. I.
    J Mol Biol. 1961 Apr;3:144-55 PMID: 13764647
  6. Hybridization between Escherichia coli and Shigella.
    J Bacteriol. 1957 Oct;74(4):461-76 PMID: 13475269
  7. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  8. Coordinate control of the synthesis of ribonucleoside diphosphate reductase components in Escherichia coli.
    J Bacteriol. 1977 May;130(2):957-9 PMID: 400802
  9. Regulation of ribonucleoside diphosphate reductase synthesis in Escherichia coli: increased enzyme synthesis as a result of inhibition of deoxyribonucleic acid synthesis.
    J Bacteriol. 1977 Apr;130(1):107-13 PMID: 67110
  10. Ribonucleoside diphosphate reductase from Escherichia coli. An immunological assay and a novel purification from an overproducing strain lysogenic for phage lambdadnrd.
    J Biol Chem. 1977 Sep 10;252(17):6132-8 PMID: 197082
  11. Physiological effects of growth of an Escherichia coli temperature-sensitive dnaZ mutant at nonpermissive temperatures.
    J Bacteriol. 1977 Oct;132(1):151-8 PMID: 334720
  12. Establishment of exponential growth after a nutritional shift-up in Escherichia coli B/r: accumulation of deoxyribonucleic acid, ribonucleic acid, and protein.
    J Bacteriol. 1977 Feb;129(2):1020-33 PMID: 320174
  13. Control of ribonucleotide reductase in mammalian cells.
    Adv Enzyme Regul. 1976;14:45-62 PMID: 987694
  14. Release of repressor control of ribonucleotide reductase by thymine starvation.
    J Biol Chem. 1965 Sep;240(9):3631-40 PMID: 4284298
  15. DNA synthesis in nucleotide-permeable Escherichia coli cells. I. Preparation and properties of ether-treated cells.
    J Mol Biol. 1971 Jun 28;58(3):739-53 PMID: 4933426
  16. Genetics and function of DNA ligase in Escherichia coli.
    J Mol Biol. 1973 Jul 15;77(4):531-47 PMID: 4353284
  17. [Effects of salts on the process of cell division in E. coli].
    C R Acad Sci Hebd Seances Acad Sci D. 1969 Mar 3;268(9):1335-8 PMID: 4977057
  18. Determination of protein: a modification of the Lowry method that gives a linear photometric response.
    Anal Biochem. 1972 Aug;48(2):422-7 PMID: 4115981
  19. The rep mutation. III. Altered structure of the replicating Escherichia coli chromosome.
    J Bacteriol. 1974 Nov;120(2):805-14 PMID: 4616950
  20. Bacterial cell division regulation: characterization of the dnaH locus of Escherichia coli.
    J Bacteriol. 1974 Aug;119(2):443-9 PMID: 4604144
  21. Replication of a specific terminal chromosome segment in Escherichia coli which is required for cell division.
    J Mol Biol. 1973 Jun 25;78(1):211-28 PMID: 4581292
  22. Pedigrees of some mutant strains of Escherichia coli K-12.
    Bacteriol Rev. 1972 Dec;36(4):525-57 PMID: 4568763
  23. Mutants of Escherichia coli B-r defective in deoxyribonucleic acid initiation: dnaI, a new gene for replication.
    J Bacteriol. 1974 Jun;118(3):783-9 PMID: 4598003
  24. Escherichia coli mutants temperature-sensitive for DNA synthesis.
    Mol Gen Genet. 1971;113(3):273-84 PMID: 4946856
  25. Effect of thymine concentration on the replication velocity of DNA in a thymineless mutant of Escherichia coli.
    Nature. 1970 Apr 11;226(5241):126-31 PMID: 4908371
  26. Escherichia coli mutants with temperature-sensitive synthesis of DNA.
    Mol Gen Genet. 1970;109(2):107-22 PMID: 4925091
  27. Chromosome replication and the division cycle of Escherichia coli B/r.
    J Mol Biol. 1968 Feb 14;31(3):519-40 PMID: 4866337
  28. The rep mutation. IV. Slower movement of replication forks in Escherichia coli rep strains.
    J Mol Biol. 1975 Sep 5;97(1):99-112 PMID: 1100854
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.135.2.429-435.1978
Published
1978-08-00
Pages
429-35
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC222400
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.135.2.429-435.1978 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2