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PMID: 3550799 Published · ppublish English Journal Article

Macromolecular crowding increases binding of DNA polymerase to DNA: an adaptive effect.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 7 ·1987-04-00 ·Pages 1871-5

Zimmerman SB, Harrison B

Abstract

Macromolecular crowding extends the range of ionic conditions supporting high DNA polymerase reaction rates. Reactions tested were nick-translation and gap-filling by DNA polymerase I of Escherichia coli, nuclease and polymerase activities of the large fragment of that polymerase, and polymerization by the T4 DNA polymerase. For all of these reactions, high concentrations of nonspecific polymers increased enzymatic activity under otherwise inhibitory conditions resulting from relatively high ionic strength. The primary mechanism of the polymer effect seems to be to increase the binding of polymerase to DNA. We suggest that this effect on protein-DNA complexes is only one example of a general "metabolic buffering" action of crowded solutions on a variety of macromolecular interactions.

MeSH Terms
Binding Sites DNA Polymerase I/metabolism DNA-Directed DNA Polymerase/metabolism Escherichia coli/enzymology Kinetics Polydeoxyribonucleotides/metabolism Polyethylene Glycols/pharmacology Protein Binding Protein Biosynthesis T-Phages/enzymology
Chemicals
Polydeoxyribonucleotides Polyethylene Glycols DNA Polymerase I DNA-Directed DNA Polymerase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Zimmerman S B
Harrison B
References (28)
28 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.7.1871
Published
1987-04-00
Pages
1871-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC304543
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.84.7.1871 PMC Full Text View journal details
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