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PMID: 3546376 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Comparative effects in vivo of recombinant murine interleukin 3, natural murine colony-stimulating factor-1, and recombinant murine granulocyte-macrophage colony-stimulating factor on myelopoiesis in mice.

The Journal of clinical investigation ·Vol. 79 ·No. 3 ·1987-03-00 ·Pages 721-30

Broxmeyer HE, Williams DE, Cooper S, Shadduck RK, Gillis S, Waheed A, Urdal DL, Bicknell DC

Abstract

Purified murine colony-stimulating factors (CSF) recombinant interleukin 3 (IL-3), natural CSF-1, and recombinant granulocyte-macrophage (GM) CSF were assessed in vivo for their effects on BDF1 mouse bone marrow and spleen granulocyte-macrophage (CFU-GM), erythroid (BFU-E), and multipotential (CFU-GEMM) progenitor cells in untreated mice and in mice pretreated with purified iron-saturated human lactoferrin (LF). The CSF and LF preparations did not contain detectable endotoxin (less than 0.1 ng). Mice pretreated with LF were more sensitive to the effects of CSF. In mice pretreated with LF, 2,000 U IL-3 or 20,000 U CSF-1 significantly enhanced the cycling status and absolute numbers of all progenitors, whereas 20,000 U GM-CSF significantly increased the cycling status of CFU-GM and CFU-GEMM, but had no effect on cycling of BFU-E or on numbers of any of the progenitors. The effects of CSF in mice pretreated with LF were not mimicked by 0.1-100 ng E. coli lipopolysaccharide.

MeSH Terms
Animals Bone Marrow Cells Cells, Cultured Colony-Stimulating Factors/pharmacology Escherichia coli Granulocytes Hematopoiesis Hematopoietic Stem Cells/cytology Interleukin-3/physiology Lactoferrin/pharmacology Lipopolysaccharides/pharmacology Mice Mice, Inbred C57BL Mice, Inbred DBA Recombinant Proteins Spleen/cytology
Chemicals
Colony-Stimulating Factors Interleukin-3 Lipopolysaccharides Recombinant Proteins Lactoferrin
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Broxmeyer H E
Williams D E
Cooper S
Shadduck R K
Gillis S
Waheed A
Urdal D L
Bicknell D C
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI112877
Published
1987-03-00
Pages
721-30
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC424183
Subset
IM
Grants
NCI NIH HHS · CA-15237 · United States
NCI NIH HHS · CA-36464 · United States
NCI NIH HHS · CA-36740 · United States
External Links
PubMed source DOI: 10.1172/JCI112877 PMC Full Text View journal details
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