Home LiteratureArticle Details
PMID: 3458209 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Pluripoietin alpha: a second human hematopoietic colony-stimulating factor produced by the human bladder carcinoma cell line 5637.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 8 ·1986-04-00 ·Pages 2478-82

Gabrilove JL, Welte K, Harris P, Platzer E, Lu L, Levi E, Mertelsmann R, Moore MA

Abstract

We have identified a factor constitutively produced by the human bladder carcinoma cell line 5637, which has the following capacities: to induce the differentiation of the human promyelocytic leukemic cell line HL-60; to induce the expression of chemotactic peptide receptors in leukemic cells as well as in normal peripheral blood granulocytes; to function as a chemoattractant for neutrophils as well as, under certain conditions, inhibiting their migration; to support the growth of eosinophil and granulocyte/macrophage progenitors, which is maximal when scored at day 14; and to support the growth of human mixed pluripotent progenitors and erythroid bursts from normal human bone marrow. This factor has a molecular size of 32 kDa by gel filtration and 16.5 kDa by NaDodSO4/PAGE. We have termed this factor pluripoietin alpha.

MeSH Terms
Bone Marrow Cells Carcinoma/analysis Cell Differentiation Cell Line Chemotaxis, Leukocyte Erythropoiesis Growth Substances/isolation & purification,physiology Hematopoiesis Hematopoietic Stem Cells/cytology Humans Molecular Weight Neoplasm Proteins/isolation & purification,physiology Neoplastic Stem Cells/cytology,physiology Receptors, Formyl Peptide Receptors, Immunologic/metabolism Urinary Bladder Neoplasms/analysis
Chemicals
Growth Substances Neoplasm Proteins Receptors, Formyl Peptide Receptors, Immunologic
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Gabrilove J L
Welte K
Harris P
Platzer E
Lu L
Levi E
Mertelsmann R
Moore M A
References (17)
17 references, click to expand
  1. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  2. Chemotaxis of monocytes and neutrophils to platelet-derived growth factor.
    J Clin Invest. 1982 Apr;69(4):1046-9 PMID: 7076844
  3. Isolation of lymphocytes, granulocytes and macrophages.
    Scand J Immunol. 1976 Jun;Suppl 5:9-15 PMID: 1052391
  4. Separation of functionally distinct human granulocyte-macrophage colony-stimulating factors.
    Blood. 1979 Sep;54(3):614-27 PMID: 313822
  5. Clonal extinction of myelomonocytic leukemic cells by serum from mice injected with endotoxin.
    Int J Cancer. 1980 Feb 15;25(2):225-33 PMID: 6967053
  6. Clonal analysis of the response of HL60 human myeloid leukemia cells to biological regulators.
    Leuk Res. 1983;7(2):117-32 PMID: 6602251
  7. A cell line secreting stimulating factors for CFU-GEMM culture.
    Blood. 1984 Jul;64(1):152-5 PMID: 6733267
  8. Purified human granulocyte-macrophage colony-stimulating factor: direct action on neutrophils.
    Science. 1984 Dec 14;226(4680):1339-42 PMID: 6390681
  9. Synergism between hemopoietic growth factors (HGFs) detected by their effects on cells bearing receptors for a lineage specific HGF: assay of hemopoietin-1.
    J Cell Physiol. 1985 Mar;122(3):370-8 PMID: 2981896
  10. Purification and biochemical characterization of human pluripotent hematopoietic colony-stimulating factor.
    Proc Natl Acad Sci U S A. 1985 Mar;82(5):1526-30 PMID: 3871951
  11. Human granulocyte-macrophage colony-stimulating factor is a neutrophil activator.
    Nature. 1985 Mar 28-Apr 3;314(6009):361-3 PMID: 2984574
  12. Purification of hemopoietin 1: a multilineage hemopoietic growth factor.
    Proc Natl Acad Sci U S A. 1985 May;82(9):2764-8 PMID: 2986136
  13. Human GM-CSF: molecular cloning of the complementary DNA and purification of the natural and recombinant proteins.
    Science. 1985 May 17;228(4701):810-5 PMID: 3923623
  14. Distinct differentiation-inducing activities of gamma-interferon and cytokine factors acting on the human promyelocytic leukemia cell line HL-60.
    Cancer Res. 1985 Jul;45(7):3090-5 PMID: 2988760
  15. Biological activities of a human pluripotent hemopoietic colony stimulating factor on normal and leukemic cells.
    J Exp Med. 1985 Dec 1;162(6):1788-801 PMID: 2999287
  16. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  17. An endotoxin-induced serum factor that causes necrosis of tumors.
    Proc Natl Acad Sci U S A. 1975 Sep;72(9):3666-70 PMID: 1103152
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.8.2478
Published
1986-04-00
Pages
2478-82
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC323321
Subset
IM
Grants
NCI NIH HHS · CA 00966 · United States
NCI NIH HHS · CA 20194 · United States
NCI NIH HHS · CA 23766 · United States
External Links
PubMed source DOI: 10.1073/pnas.83.8.2478 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2