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PMID: 3539799 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Functional characterization of extracellular vesicles produced by Bacteroides gingivalis.

Infection and immunity ·Vol. 55 ·No. 1 ·1987-01-00 ·Pages 111-7

Grenier D, Mayrand D

Abstract

Extracellular vesicles of Bacteroides gingivalis (type strain 33277) were isolated, and some of their biological activities were characterized. The vesicles were obtained from a 2-day culture after ammonium sulfate precipitation, differential centrifugation, and dialysis. When viewed by electron microscopy, vesicles of approximately 50 nm predominated. The results indicated that the enriched vesicle fraction had a high proteolytic activity against collagen, Azocoll, and N-alpha-benzoyl-DL-arginine p-nitroanilide. The polypeptide pattern of the vesicles was similar but not identical to that of the outer membrane. The membrane vesicles could also promote bacterial adherence between homologous cells as well as mediate attachment between two noncoaggregating bacterial species. These vesicles could thus play an important role in periodontal diseases by serving as a vehicle for toxins and various proteolytic enzymes, as well as being involved in adherence.

MeSH Terms
Bacterial Adhesion Bacteroides/physiology,ultrastructure Cell Fractionation Cell Membrane/enzymology Erythrocytes/microbiology Hydrogen-Ion Concentration Microscopy, Electron Peptide Hydrolases/metabolism
Chemicals
Peptide Hydrolases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Grenier D
Mayrand D
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.55.1.111-117.1987
Published
1987-01-00
Pages
111-7
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC260287
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.55.1.111-117.1987 PMC Full Text View journal details
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