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PMID: 6746097 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Fractionation of hemagglutinating and bacterial binding adhesins of Bacteroides gingivalis.

Infection and immunity ·Vol. 45 ·No. 2 ·1984-08-00 ·Pages 403-9

Boyd J, McBride BC

Abstract

An outer membrane complex containing hemagglutinating and bacterial aggregating activity has been isolated from Bacteroides gingivalis. Examination of the membrane material by biochemical analysis, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and immunological means revealed that the crude outer membrane preparation contained three major proteins and a lipopolysaccharide population that displayed size heterogeneity. At least two membrane proteins as well as the lipopolysaccharide were found to be antigenically active by immunoblot analysis. With gel chromatography and a lipopolysaccharide disaggregating buffer the membrane material was separated into two fractions. An accompanying separation of the two adherence activities was observed. The first membrane fraction, containing mostly protein and carbohydrate material, was found to contain the bacterial aggregating activity. This fraction also contained a high-molecular-weight lipopolysaccharide population. The second membrane fraction, consisting of low-molecular-weight lipopolysaccharide, protein, and loosely bound lipid was found to contain the hemagglutinating activity.

MeSH Terms
Adhesiveness Antigens, Bacterial/analysis Bacterial Outer Membrane Proteins Bacterial Proteins/analysis Bacteroides/analysis,ultrastructure Hemagglutinins/analysis Humans Membrane Proteins/analysis Microscopy, Electron Periodontitis/microbiology Polysaccharides, Bacterial/analysis
Chemicals
Antigens, Bacterial Bacterial Outer Membrane Proteins Bacterial Proteins Hemagglutinins Membrane Proteins Polysaccharides, Bacterial
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Boyd J
McBride B C
References (31)
31 references, click to expand
  1. Purification and properties of C 55 -isoprenoid alcohol phosphokinase from Staphylococcus aureus.
    J Biol Chem. 1972 Aug 25;247(16):5123-31 PMID: 5057461
  2. Studies on the chemical structure of the streptococcal cell wall. I. The identification of a mucopeptide in the cell walls of groups A and A-variant streptococci.
    J Exp Med. 1961 Jul 1;114:127-40 PMID: 13754097
  3. Protein composition of the outer membrane of Salmonella typhimurium: effect of lipopolysaccharide mutations.
    J Bacteriol. 1974 Feb;117(2):406-16 PMID: 4590466
  4. Haemagglutinating activity of Bacteroides melaninogenicus.
    Arch Oral Biol. 1974 May;19(5):415-6 PMID: 4527823
  5. Immunochemical characterization of the outer membrane complex of Bacteroides fragilis subspecies fragilis.
    J Infect Dis. 1975 Oct;132(4):440-50 PMID: 810521
  6. Heterogeneity of lipopolysaccharides. Analysis of polysaccharide chain lengths by sodium dodecylsulfate-polyacrylamide gel electrophoresis.
    Eur J Biochem. 1975 Dec 1;60(1):239-46 PMID: 1107034
  7. Electrophoretic resolution of the "major outer membrane protein" of Escherichia coli K12 into four bands.
    FEBS Lett. 1975 Oct 15;58(1):254-8 PMID: 773686
  8. Outer membrane of Salmonella. Isolation of protein complex that produces transmembrane channels.
    J Biol Chem. 1976 Apr 10;251(7):2176-8 PMID: 773934
  9. Identification of the outer membrane protein of E. coli that produces transmembrane channels in reconstituted vesicle membranes.
    Biochem Biophys Res Commun. 1976 Aug 9;71(3):877-84 PMID: 786294
  10. Effects of heating in dodecyl sulfate solution on the conformation and electrophoretic mobility of isolated major outer membrane proteins from Escherichia coli K-12.
    J Biochem. 1976 Dec;80(6):1411-22 PMID: 828162
  11. Purification and immunochemical characterization of the outer membrane complex of Bacteroides melaninogenicus subspecies asaccharolyticus.
    J Infect Dis. 1977 May;135(5):787-99 PMID: 16065
  12. Reconstitution of model membranes from phospholipid and outer membrane proteins of Proteus mirabilis. Role of proteins in the formation of hydrophilic pores and protection of membranes against detergents.
    Eur J Biochem. 1977 Nov 15;81(1):63-9 PMID: 338302
  13. Attachment of Bacteroides melaninogenicus subsp. asaccharolyticus to oral surfaces and its possible role in colonization of the mouth and of periodontal pockets.
    Infect Immun. 1978 Jan;19(1):254-64 PMID: 24002
  14. Identification of a subspecies-specific capsular antigen from Bacteroides melaninogenicus subspecies asaccharolyticus by immunofluorescence and electron microscopy.
    J Infect Dis. 1978 Dec;138(6):736-41 PMID: 84036
  15. Ultrastructure of Bacteroides species: Bacteroides asaccharolyticus, Bacteroides fragilis, Bacteroides melaninogenicus subspecies melaninogenicus, and B. melaninogenicus subspecies intermedius.
    J Infect Dis. 1979 May;139(5):534-46 PMID: 86590
  16. Immunological study of the pili of Bacteroides melaninogenicus.
    Bull Tokyo Dent Coll. 1978 May;19(2):93-5 PMID: 292529
  17. Immunochemical differences between oral and nonoral strains of Bacteroides asaccharolyticus.
    Infect Immun. 1980 Feb;27(2):589-96 PMID: 7380540
  18. Immunochemical characterization of surface antigens of Bacteroides melaninogenicus.
    Rev Infect Dis. 1979 Mar-Apr;1(2):263-77 PMID: 44922
  19. Heterogeneity of antigenic-side-chain length in lipopolysaccharide from Escherichia coli 0111 and Salmonella typhimurium LT2.
    Eur J Biochem. 1980;107(1):145-53 PMID: 6156828
  20. Isolation of two protein-free and chemically different lipopolysaccharides from Bordetella pertussis phenol-extracted endotoxin.
    J Bacteriol. 1980 Jul;143(1):78-88 PMID: 6249793
  21. A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels.
    Anal Biochem. 1980 Jul 1;105(2):361-3 PMID: 6161559
  22. Characterization of the adherence properties of Streptococcus salivarius.
    Infect Immun. 1980 Aug;29(2):459-68 PMID: 7216420
  23. Antigenic studies of oral and nonoral black-pigmented Bacteroides strains.
    Infect Immun. 1980 Aug;29(2):564-74 PMID: 7216426
  24. A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels.
    Anal Biochem. 1982 Jan 1;119(1):115-9 PMID: 6176137
  25. Chemical and chromatographic analysis of lipopolysaccharide from an antibiotic-supersusceptible mutant of Pseudomonas aeruginosa.
    Antimicrob Agents Chemother. 1982 Feb;21(2):310-9 PMID: 6803667
  26. Ultrastructure of the outer membrane surface of black-pigmented Bacteroides isolated from human oral cavity.
    Bull Tokyo Dent Coll. 1982 Feb;23(1):47-60 PMID: 6125273
  27. Procedure for isolation of bacterial lipopolysaccharides from both smooth and rough Pseudomonas aeruginosa and Salmonella typhimurium strains.
    J Bacteriol. 1983 Aug;155(2):831-8 PMID: 6409884
  28. Proteinaceous bacterial adhesins and their receptors.
    Crit Rev Microbiol. 1983;10(3):229-60 PMID: 6138214
  29. The determination of sugar in blood and spinal fluid with anthrone reagent.
    J Biol Chem. 1955 Jan;212(1):335-43 PMID: 13233235
  30. A rapid method of total lipid extraction and purification.
    Can J Biochem Physiol. 1959 Aug;37(8):911-7 PMID: 13671378
  31. New method for quantitative determination of uronic acids.
    Anal Biochem. 1973 Aug;54(2):484-9 PMID: 4269305
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.45.2.403-409.1984
Published
1984-08-00
Pages
403-9
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC263237
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.45.2.403-409.1984 PMC Full Text View journal details
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