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PMID: 3536846 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Glutathione in Escherichia coli is dispensable for resistance to H2O2 and gamma radiation.

Journal of bacteriology ·Vol. 168 ·No. 2 ·1986-11-00 ·Pages 1026-9

Greenberg JT, Demple B

Abstract

Escherichia coli devoid of glutathione (because of transposon insertions in the gshA gene) has normal resistance to H2O2, cumene hydroperoxide, heat, or ionizing radiation. Intracellular glutathione thus does not protect E. coli from such lethal oxidative damage. The use of gshA::Tn10 mutants also revealed a glutathione-independent, H2O2-inducible resistance to N-ethylmaleimide.

MeSH Terms
Benzene Derivatives/pharmacology Drug Resistance, Microbial Escherichia coli/drug effects,genetics,physiology,radiation effects Ethylmaleimide/pharmacology Gamma Rays Glutathione/physiology Hot Temperature Hydrogen Peroxide/pharmacology Mutation
Chemicals
Benzene Derivatives Hydrogen Peroxide Glutathione Ethylmaleimide cumene hydroperoxide
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Greenberg J T
Demple B
References (21)
21 references, click to expand
  1. Dimethyl sulfoxide prevents DNA nicking mediated by ionizing radiation or iron/hydrogen peroxide-generated hydroxyl radical.
    Proc Natl Acad Sci U S A. 1981 Feb;78(2):1001-3 PMID: 6940118
  2. Radiosensitization of hypoxic tumor cells by depletion of intracellular glutathione.
    Science. 1982 Aug 6;217(4559):544-5 PMID: 7089580
  3. Methylation of deoxyribonucleic acid in cultured mammalian cells by N-methyl-N'-nitro-N-nitrosoguanidine. The influence of cellular thiol concentrations on the extent of methylation and the 6-oxygen atom of guanine as a site of methylation.
    Biochem J. 1970 Feb;116(4):693-707 PMID: 5435496
  4. Linkage map of Escherichia coli K-12, edition 7.
    Microbiol Rev. 1983 Jun;47(2):180-230 PMID: 6348505
  5. Positive control of a regulon for defenses against oxidative stress and some heat-shock proteins in Salmonella typhimurium.
    Cell. 1985 Jul;41(3):753-62 PMID: 2988786
  6. Role of oxygen in modifying the radiosensitivity of E. coli B.
    Nature. 1956 Nov 3;178(4540):978-9 PMID: 13378491
  7. Cellular glutathione is a key to the oxygen effect in radiation damage.
    Nature. 1978 Feb 16;271(5646):660-2 PMID: 342974
  8. Glutathione depletion, radiosensitization, and misonidazole potentiation in hypoxic Chinese hamster ovary cells by buthionine sulfoximine.
    Radiat Res. 1984 May;98(2):370-80 PMID: 6539482
  9. Mapping of gshA, a gene for the biosynthesis of glutathione in Eschericha coli K12.
    Mol Gen Genet. 1975 Nov 24;141(2):91-5 PMID: 1107813
  10. Escherichia coli xth mutants are hypersensitive to hydrogen peroxide.
    J Bacteriol. 1983 Feb;153(2):1079-82 PMID: 6337115
  11. Alkylation and oxidative damages to DNA: constitutive and inducible repair systems.
    Basic Life Sci. 1986;39:205-17 PMID: 3533043
  12. Inducible repair of oxidative DNA damage in Escherichia coli.
    Nature. 1983 Aug 4-10;304(5925):466-8 PMID: 6348554
  13. Induction and repair of single-strand DNA breaks after X-irradiation of human fibroblasts deficient in glutathione.
    Int J Radiat Biol Relat Stud Phys Chem Med. 1981 Oct;40(4):355-63 PMID: 6975250
  14. Isolation of mutants of Escherichia coli with increased resistance to alkylating agents: mutants deficient in thiols and mutants constitutive for the adaptive response.
    Mol Gen Genet. 1980;180(1):85-90 PMID: 7003313
  15. Isolation and initial characterization of glutathione-deficient mutants of Escherichia coli K 12.
    Biochim Biophys Acta. 1975 Jul 14;399(1):10-22 PMID: 1096956
  16. Isolation of an Escherichia coli mutant deficient in glutathione synthesis.
    J Bacteriol. 1975 Oct;124(1):140-8 PMID: 1100598
  17. Isolation of catalase-deficient Escherichia coli mutants and genetic mapping of katE, a locus that affects catalase activity.
    J Bacteriol. 1984 Feb;157(2):622-6 PMID: 6319370
  18. Glutathione.
    Annu Rev Biochem. 1983;52:711-60 PMID: 6137189
  19. Glutathione dependence of neocarzinostatin cytotoxicity and mutagenicity in Chinese hamster V-79 cells.
    Cancer Res. 1985 Oct;45(10):4760-2 PMID: 3161610
  20. New Tn10 derivatives for transposon mutagenesis and for construction of lacZ operon fusions by transposition.
    Gene. 1984 Dec;32(3):369-79 PMID: 6099322
  21. Endonuclease-resistant apyrimidinic sites formed by neocarzinostatin at cytosine residues in DNA: evidence for a possible role in mutagenesis.
    Proc Natl Acad Sci U S A. 1985 May;82(10):3182-6 PMID: 2582408
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.168.2.1026-1029.1986
Published
1986-11-00
Pages
1026-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC213589
Subset
IM
Grants
NCI NIH HHS · CA37831 · United States
External Links
PubMed source DOI: 10.1128/jb.168.2.1026-1029.1986 PMC Full Text View journal details
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