Home LiteratureArticle Details
PMID: 3534880 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Two mammalian heat shock proteins, HSP90 and HSP100, are actin-binding proteins.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 21 ·1986-11-00 ·Pages 8054-8

Koyasu S, Nishida E, Kadowaki T, Matsuzaki F, Iida K, Harada F, Kasuga M, Sakai H, Yahara I

Abstract

Two high molecular weight heat shock proteins, HSP90 (Mr, 90,000) and HSP100 (Mr, 100,000), were separately purified from extracts of cultured cells of a mouse lymphoma cell line, L5178Y. Both of the HSPs exist in homodimeric form under physiological conditions. Their physicochemical properties are quite similar to each other. Each of the purified HSPs was shown to coprecipitate with rabbit skeletal muscle actin under actin-polymerizing conditions. Both HSP90 and HSP100 increased the low-shear viscosity of filamentous actin solutions in a dose-dependent manner, which suggests that these HSPs cross-link actin filaments. Although some molecular properties and the effects described above on actin solution of HSP90 and HSP100 resemble those of alpha-actinin, the HSPs were distinguished from alpha-actinin by various means, including visualization of molecular shapes by electron microscopy with the aid of the low-angle rotary shadowing technique. Immunofluorescence staining by specific antisera against HSP90 revealed that HSP90 was localized in ruffling membranes in addition to the cytoplasmic space.

MeSH Terms
Actins/metabolism Animals Biological Transport Carrier Proteins/analysis Fluorescent Antibody Technique Gelsolin Heat-Shock Proteins/analysis,isolation & purification,physiology Mice Microfilament Proteins Microscopy, Electron Receptors, Steroid/metabolism
Chemicals
Actins Carrier Proteins Gelsolin Heat-Shock Proteins Microfilament Proteins Receptors, Steroid brevin
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Koyasu S
Nishida E
Kadowaki T
Matsuzaki F
Iida K
Harada F
Kasuga M
Sakai H
Yahara I
References (39)
39 references, click to expand
  1. Biochemical characterization of the mammalian stress proteins and identification of two stress proteins as glucose- and Ca2+-ionophore-regulated proteins.
    J Biol Chem. 1983 Jun 10;258(11):7102-11 PMID: 6406494
  2. Association of the transforming proteins of Rous, Fujinami, and Y73 avian sarcoma viruses with the same two cellular proteins.
    Mol Cell Biol. 1982 Jul;2(7):875-80 PMID: 6100915
  3. Insulin-induced formation of ruffling membranes of KB cells and its correlation with enhancement of amino acid transport.
    J Cell Biol. 1984 Mar;98(3):801-9 PMID: 6321519
  4. Nuclear and nucleolar localization of the 72,000-dalton heat shock protein in heat-shocked mammalian cells.
    J Biol Chem. 1984 Apr 10;259(7):4501-13 PMID: 6368558
  5. Durable synthesis of high molecular weight heat shock proteins in G0 cells of the yeast and other eucaryotes.
    J Cell Biol. 1984 Jul;99(1 Pt 1):199-207 PMID: 6429154
  6. A short sequence in the p60src N terminus is required for p60src myristylation and membrane association and for cell transformation.
    Mol Cell Biol. 1984 Sep;4(9):1834-42 PMID: 6092942
  7. Isolation and some properties of macrophage alpha-actinin: evidence that it is not an actin gelling protein.
    Biochemistry. 1984 Oct 9;23(21):5081-6 PMID: 6498177
  8. Cofilin, a protein in porcine brain that binds to actin filaments and inhibits their interactions with myosin and tropomyosin.
    Biochemistry. 1984 Oct 23;23(22):5307-13 PMID: 6509022
  9. Hsp70 accelerates the recovery of nucleolar morphology after heat shock.
    EMBO J. 1984 Dec 20;3(13):3095-100 PMID: 6441707
  10. Ubiquitin is a heat shock protein in chicken embryo fibroblasts.
    Mol Cell Biol. 1985 May;5(5):949-56 PMID: 2987683
  11. Evidence that the 90-kDa phosphoprotein associated with the untransformed L-cell glucocorticoid receptor is a murine heat shock protein.
    J Biol Chem. 1985 Oct 15;260(23):12398-401 PMID: 3900074
  12. Morphological study of the mammalian stress response: characterization of changes in cytoplasmic organelles, cytoskeleton, and nucleoli, and appearance of intranuclear actin filaments in rat fibroblasts after heat-shock treatment.
    J Cell Biol. 1985 Oct;101(4):1198-211 PMID: 3900086
  13. A 90,000-dalton binding protein common to both steroid receptors and the Rous sarcoma virus transforming protein, pp60v-src.
    J Biol Chem. 1985 Nov 15;260(26):14292-6 PMID: 2414293
  14. Interaction of actinogelin with actin. No nucleation but high gelation activity.
    Eur J Biochem. 1985 Dec 16;153(3):609-20 PMID: 4076192
  15. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  16. The regulation of rabbit skeletal muscle contraction. I. Biochemical studies of the interaction of the tropomyosin-troponin complex with actin and the proteolytic fragments of myosin.
    J Biol Chem. 1971 Aug 10;246(15):4866-71 PMID: 4254541
  17. Actin antibody: the specific visualization of actin filaments in non-muscle cells.
    Proc Natl Acad Sci U S A. 1974 Jun;71(6):2268-72 PMID: 4210210
  18. Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.
    J Biol Chem. 1977 Feb 10;252(3):1102-6 PMID: 320200
  19. High resolution two-dimensional electrophoresis of basic as well as acidic proteins.
    Cell. 1977 Dec;12(4):1133-41 PMID: 23215
  20. Immunofluorescence on avian sarcoma virus-transformed cells: localization of the src gene product.
    Cell. 1979 Jan;16(1):11-24 PMID: 217542
  21. The induction of gene activity in drosophilia by heat shock.
    Cell. 1979 Jun;17(2):241-54 PMID: 110462
  22. Ca2+-sensitive gelation of actin filaments by a new protein factor.
    Nature. 1979 Nov 1;282(5734):44-8 PMID: 503187
  23. Fluorescent phallotoxin, a tool for the visualization of cellular actin.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4498-502 PMID: 291981
  24. The common 90-kd protein component of non-transformed '8S' steroid receptors is a heat-shock protein.
    EMBO J. 1985 Dec 1;4(12):3131-5 PMID: 2419124
  25. A rapid purification of alpha-actinin, filamin, and a 130,000-dalton protein from smooth muscle.
    J Biol Chem. 1980 Feb 10;255(3):1194-9 PMID: 7356657
  26. Viscometric analysis of the gelation of Acanthamoeba extracts and purification of two gelation factors.
    J Cell Biol. 1980 May;85(2):414-28 PMID: 6892818
  27. Rotary shadowing of extended molecules dried from glycerol.
    J Ultrastruct Res. 1980 May;71(2):95-102 PMID: 6155474
  28. Heat shock proteins are methylated in avian and mammalian cells.
    Proc Natl Acad Sci U S A. 1981 Jun;78(6):3531-5 PMID: 6943552
  29. Two cellular proteins that immunoprecipitate with the transforming protein of Rous sarcoma virus.
    Virology. 1981 Sep;113(2):736-51 PMID: 6267806
  30. The specific interaction of the Rous sarcoma virus transforming protein, pp60src, with two cellular proteins.
    Cell. 1981 Aug;25(2):363-72 PMID: 6269742
  31. Non-muscle alpha actinins are calcium-sensitive actin-binding proteins.
    Nature. 1981 Dec 10;294(5841):565-7 PMID: 7312045
  32. The transforming proteins of PRCII virus and Rous sarcoma virus form a complex with the same two cellular phosphoproteins.
    J Virol. 1982 Aug;43(2):448-55 PMID: 6180178
  33. Antibodies to two major chicken heat shock proteins cross-react with similar proteins in widely divergent species.
    Mol Cell Biol. 1982 Mar;2(3):267-74 PMID: 7110134
  34. Purification of the major mammalian heat shock proteins.
    J Biol Chem. 1982 Dec 25;257(24):14949-59 PMID: 7174676
  35. The 70-kd mammalian heat shock proteins are structurally and functionally related to the uncoating protein that releases clathrin triskelia from coated vesicles.
    EMBO J. 1985 Dec 16;4(13A):3385-91 PMID: 2868889
  36. A heat shock-resistant variant of Chinese hamster cell line constitutively expressing heat shock protein of Mr 90,000 at high level.
    Cell Struct Funct. 1986 Mar;11(1):65-73 PMID: 3955665
  37. Reversible induction of actin rods in mouse C3H-2K cells by incubation in salt buffers and by treatment with non-ionic detergents.
    Exp Cell Res. 1986 Jun;164(2):492-506 PMID: 3086110
  38. Heat shock induction of intranuclear actin rods in cultured mammalian cells.
    Exp Cell Res. 1986 Jul;165(1):207-15 PMID: 3519257
  39. hsp70: nuclear concentration during environmental stress and cytoplasmic storage during recovery.
    Cell. 1984 Mar;36(3):655-62 PMID: 6421488
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.21.8054
Published
1986-11-00
Pages
8054-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC386865
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.83.21.8054 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2