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PMID: 291981 Published · ppublish English Journal Article

Fluorescent phallotoxin, a tool for the visualization of cellular actin.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 9 ·1979-09-00 ·Pages 4498-502

Wulf E, Deboben A, Bautz FA, Faulstich H, Wieland T

Abstract

A fluorescent derivative of phalloidin has been synthesized possessing high affinity to filamentous actin. This compound was used for visualization of actin-containing structures in eukaryotic nonmuscle cells. Due to its low molecular weight (1250), fixation for formaldehyde was sufficient to render the membrane permeable for the labeled peptide. Bundles of microfilaments are the predominant pattern in the flat rat kangaroo PtK1 cells, whereas a net of concentric fibers characterizes the more spherical bovine kidney MDBK cells. Specificity of staining was confirmed by competition experiments with unlabeled phalloidin.

MeSH Terms
Actins/metabolism Animals Cattle Cell Line Cytoskeleton/ultrastructure Fluorescent Dyes Kidney Macropodidae Microscopy, Fluorescence Mitosis Oligopeptides/metabolism Phalloidine/analogs & derivatives,metabolism
Chemicals
Actins Fluorescent Dyes Oligopeptides Phalloidine
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Wulf E
Deboben A
Bautz F A
Faulstich H
Wieland T
References (24)
24 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.9.4498
Published
1979-09-00
Pages
4498-502
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC411604
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.9.4498 PMC Full Text View journal details
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