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PMID: 3529083 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Isolation of a cDNA clone encoding the leader peptide of prion protein and expression of the homologous gene in various tissues.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 17 ·1986-09-00 ·Pages 6377-81

Robakis NK, Sawh PR, Wolfe GC, Rubenstein R, Carp RI, Innis MA

Abstract

We have isolated a hamster cDNA clone representing the coding sequences for the entire precursor of prion protein (PrP) 27-30. This clone encodes a protein of 254 residues and contains an in-frame ATG codon 42 bases upstream from the one previously reported. Analysis of the predicted amino acid sequence suggests that the PrP precursor protein contains an amino-terminal signal sequence, and a membrane-spanning domain in the carboxyl terminus. Cleavage of the signal peptide would produce a mature protein of 232 amino acids. Sequences homologous to the hamster PrP cDNA were detected in hamster, mouse, sheep, human, and rabbit genomes. A related 2.5-kilobase transcript was present in the brain of normal and scrapie-infected rodents. Two homologous transcripts, 2.5 and 1.1 kilobases, were detected in the lung and heart of normal animals. No PrP mRNA was detected in spleen stroma, a tissue known to contain high titers of scrapie. Antisera raised to the 27- to 30-kDa polypeptide detected the PrP in both normal and infected brains but failed to detect this protein in either normal or infected spleens. Homologous mRNA species were detected in human, sheep, and rabbit brain, even though the latter is resistant to scrapie infection. Our data suggest that PrP is not a necessary component of the infectious agent.

MeSH Terms
Amino Acid Sequence Animals Cloning, Molecular Cricetinae DNA/genetics Gene Expression Regulation Immunosorbent Techniques Prions/genetics,immunology Protein Conformation Protein Precursors/genetics RNA, Messenger/genetics Solubility Species Specificity Tissue Distribution
Chemicals
Prions Protein Precursors RNA, Messenger DNA
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Robakis N K
Sawh P R
Wolfe G C
Rubenstein R
Carp R I
Innis M A
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.17.6377
Published
1986-09-00
Pages
6377-81
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC386506
Subset
IM
Grants
NIA NIH HHS · AG04220 · United States
NINDS NIH HHS · NS21349 · United States
External Links
PubMed source DOI: 10.1073/pnas.83.17.6377 PMC Full Text View journal details
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