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PMID: 3520551 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Cellular myc (c-myc) in fish (rainbow trout): its relationship to other vertebrate myc genes and to the transforming genes of the MC29 family of viruses.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 11 ·1986-06-00 ·Pages 3698-702

Van Beneden RJ, Watson DK, Chen TT, Lautenberger JA, Papas TS

Abstract

We have isolated, cloned, and sequenced the rainbow trout (Salmo gairdneri) c-myc gene. The presumptive coding region of the trout c-myc gene shows extensive homology to the c-myc genes of chicken, mouse, and human. Comparison of nucleotide sequences reveals that human, mouse, chicken, and trout c-myc genes contain at least two coding exons, interrupted by introns of decreasing size of 1.38 kilobases (kb), 1.2 kb, 0.97 kb, and 0.33 kb, respectively. The exons are clearly delineated by donor-acceptor splice signals. The degree of nucleotide homology between trout, chicken, and human exon II is less than that observed for exon III. However, the greatest homology among these three genes is localized to two specific regions within exon II (myc boxes A and B). At the predicted amino acid level, fish c-myc shows considerable homology to vertebrate c-myc gene products. Trout c-myc is expressed in normal trout cells as a single 2.3-kb mRNA species, similar in size to other vertebrate transcripts.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cloning, Molecular Fishes/genetics Gene Expression Regulation Genes Oncogene Proteins, Viral/genetics Proto-Oncogene Proteins/genetics Sequence Homology, Nucleic Acid Transcription, Genetic
Chemicals
Oncogene Proteins, Viral Proto-Oncogene Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Van Beneden R J
Watson D K
Chen T T
Lautenberger J A
Papas T S
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32 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.11.3698
Published
1986-06-00
Pages
3698-702
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC323590
Subset
IM
Databases
GENBANK
M13048
External Links
PubMed source DOI: 10.1073/pnas.83.11.3698 PMC Full Text View journal details
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