Artificially induced active transport of amino acid driven by the efflux of a sugar via a heterologous transport system in de-energized Escherichia coli.

The Biochemical journal ·Vol. 178 ·No. 1 ·1979-01-15 ·Pages 103-7

Bentaboulet M, Robin A, Kepes A

Abstract

Consistent with the model of an H+ cotransport, amino acid uptake can be driven by a proton gradient generated by an efflux of sugar when the normal energy sources are suppressed. Heterologous countertransport is completely inhibited by uncouplers unlike homologous countertransport. Positive coupling was obtained with methyl thiogalactoside/proline, methyl thiogalactoside/phenylalanine, gluconate/proline; however, the poor coupling efficiency suggests a more complex sequence of reactions.

MeSH Terms

Amino Acids/metabolism Biological Transport, Active/drug effects Carbohydrate Metabolism Carbonyl Cyanide m-Chlorophenyl Hydrazone/pharmacology Electrochemistry Escherichia coli/drug effects,metabolism Gluconates/metabolism Hydrogen-Ion Concentration Phenylalanine/metabolism Proline/metabolism Thiogalactosides/metabolism

Chemicals

Amino Acids Gluconates Thiogalactosides Phenylalanine Carbonyl Cyanide m-Chlorophenyl Hydrazone Proline

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Bentaboulet M

Robin A

Kepes A

References (17)

17 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1780103

Published

1979-01-15

Pages

103-7

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1186485

Subset

External Links

PubMed source DOI: 10.1042/bj1780103 PMC Full Text View journal details

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Artificially induced active transport of amino acid driven by the efflux of a sugar via a heterologous transport system in de-energized Escherichia coli.

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