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PMID: 23116 Published · ppublish English Journal Article

Evidence for an electrogenic 3-deoxy-2-oxo-D-gluconate--proton co-transport driven by the protonmotive force in Escherichia coli K12.

The Biochemical journal ·Vol. 168 ·No. 2 ·1977-11-15 ·Pages 211-21

Lagarde A

Abstract

Evidence is presented indicating that the carrier-mediated uptake of 3-deoxy-2-oxo-D-gluconate and D-glucuronate in Escherichia coli K12 is driven by the deltapH and deltapsi components of the protonmotive force. 1. Approximately two protons enter the cells with each sugar molecule, independent of the sugar and the strain used. 2. In respiring cells, the magnitude of the pH gradient alone, as measured by distribution of [3H]acetate, appears to be insufficient to account for the chemical gradient of 3-deoxy-2-oxo-D-gluconate that is developed between pH 6.0 and 8.0. 3. If the external pH is varied between 5.5 and 8.0, 3-deoxy-2-oxo-D-gluconate uptake is gradually inhibited by valinomycin plus K+ ions, whereas the inhibition caused by nigericin is concomitantly relieved, thus reflecting the relative contribution of deltapH and deltapsi to the total protonmotive force at each external pH. 4. 3-Deoxy-2-oxo-D-gluconate can be transiently accumulated into isolated membrane vesicles in response to an artificially induced pH gradient. The process is stimulated when the membrane potential is collapsed by valinomycin in the presence of K+ ions.

MeSH Terms
Biological Transport, Active Cations/metabolism Cell Membrane/metabolism Electrochemistry Escherichia coli/drug effects,metabolism Gluconates/metabolism Hydrogen-Ion Concentration Ionophores/pharmacology Kinetics Potassium/metabolism Protons
Chemicals
Cations Gluconates Ionophores Protons Potassium
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Lagarde A
References (45)
45 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1680211
Published
1977-11-15
Pages
211-21
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1183754
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1680211 PMC Full Text View journal details
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