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PMID: 3513167 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Anticodon-anticodon interaction induces conformational changes in tRNA: yeast tRNAAsp, a model for tRNA-mRNA recognition.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 4 ·1986-02-00 ·Pages 932-6

Moras D, Dock AC, Dumas P, Westhof E, Romby P, Ebel JP, Giegé R

Abstract

The crystal structure of yeast tRNAAsp enables visualization of an anticodon-anticodon interaction at the molecular level. Except for differences in the base stacking and twist, the overall conformation of the anticodon loop is quite similar to that of yeast tRNAPhe. The anticodon nucleotide triplets, GUC, of two symmetrically related molecules form a minihelix of the RNA type 11. The modified base m1G37 stacks on both sides of the triplets and enforces the continuity with the anticodon stems. Anticodon association induces long-range conformational changes in the region of the dihydrouracil and thymine loops. Experimental evidence includes the variation in the distribution of temperature factors between yeast tRNAPhe and tRNAAsp, the difference in the self-splitting patterns of tRNAAsp in crystal and solution, and the differential accessibility of cytidines to dimethyl sulfate in free and duplex tRNAAsp. These observations are linked to the fragility and disruption of the G.C Watson-Crick base pair at the corner of the molecule formed by the dihydrouracil and thymine loops.

MeSH Terms
Anticodon/metabolism Models, Molecular Nucleic Acid Conformation RNA, Fungal/metabolism RNA, Messenger/metabolism RNA, Transfer/metabolism RNA, Transfer, Amino Acyl/metabolism Saccharomyces cerevisiae/genetics X-Ray Diffraction
Chemicals
Anticodon RNA, Fungal RNA, Messenger RNA, Transfer, Amino Acyl RNA, Transfer
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Moras D
Dock A C
Dumas P
Westhof E
Romby P
Ebel J P
Giegé R
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36 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.4.932
Published
1986-02-00
Pages
932-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC322984
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.83.4.932 PMC Full Text View journal details
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