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PMID: 3510193 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Plasmid segregation into minicells is associated with membrane attachment and independent of plasmid replication.

Journal of bacteriology ·Vol. 165 ·No. 1 ·1986-01-00 ·Pages 82-7

Rashtchian A, Brown SW, Reichler J, Levy SB

Abstract

The role of plasmid replication in the segregation of plasmids into Escherichia coli minicells was investigated with temperature-sensitive replication mutants derived from E. coli plasmids ColE1 and pSC101. For as long as six generations of growth, at permissive or nonpermissive temperatures (when greater than 80% of plasmid replication was inhibited), the same amount of previously 3H-labeled plasmid DNA segregated into minicells. Density gradient separations of wild-type and temperature-sensitive plasmid DNA from both replicons segregated into the minicells showed that about 20 to 25% was stably associated with the minicell membrane at both temperatures. Electron microscopy showed this DNA to consist of circular plasmid molecules attached to the minicell membrane. These combined findings suggest that segregation of plasmids into minicells and their association with the minicell membrane are interrelated and independent of plasmid replication.

MeSH Terms
Adhesiveness Cell Membrane/metabolism DNA Replication Escherichia coli/genetics Plasmids Tritium
Chemicals
Tritium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Rashtchian A
Brown S W
Reichler J
Levy S B
References (20)
20 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.165.1.82-87.1986
Published
1986-01-00
Pages
82-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC214373
Subset
IM
Grants
NIAID NIH HHS · AI20268 · United States
External Links
PubMed source DOI: 10.1128/jb.165.1.82-87.1986 PMC Full Text View journal details
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