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PMID: 3491296 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Promoter region of interleukin-2 gene undergoes chromatin structure changes and confers inducibility on chloramphenicol acetyltransferase gene during activation of T cells.

Molecular and cellular biology ·Vol. 6 ·No. 9 ·1986-09-00 ·Pages 3042-9

Siebenlist U, Durand DB, Bressler P, Holbrook NJ, Norris CA, Kamoun M, Kant JA, Crabtree GR

Abstract

The chromatin structure of the interleukin-2 (IL-2) gene was probed by DNase I treatment of isolated nuclei. The 5' region of the IL-2 gene contains three regions of hypersensitivity to DNase I. When peripheral blood T cells or Jurkat T cells are stimulated with mitogens, IL-2 message is induced, and the promoter region of the IL-2 gene develops an additional hypersensitive site. This suggests that a DNA sequence close to the transcriptional start site is involved in the transduction of the extracellular signal. Such a conclusion is further supported by DNA transfection experiments. A short segment of DNA, which includes the region of induced hypersensitivity, confers inducibility on the linked chloramphenicol acetyltransferase gene in transiently transfected Jurkat cells. In addition, cells of nonhematopoietic origins exhibit a strikingly different chromatin pattern of IL-2, suggesting a role during differentiation for some of the hypersensitive sites.

MeSH Terms
Acetyltransferases/biosynthesis,genetics Amino Acid Sequence Base Sequence Cell Line Cells, Cultured Chloramphenicol O-Acetyltransferase Chromatin/drug effects,physiology Enzyme Induction Genes/drug effects Humans Interleukin-2/genetics Lymphocyte Activation Plasmids Promoter Regions, Genetic T-Lymphocytes/immunology Tetradecanoylphorbol Acetate/pharmacology
Chemicals
Chromatin Interleukin-2 Acetyltransferases Chloramphenicol O-Acetyltransferase Tetradecanoylphorbol Acetate
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Siebenlist U
Durand D B
Bressler P
Holbrook N J
Norris C A
Kamoun M
Kant J A
Crabtree G R
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.6.9.3042-3049.1986
Published
1986-09-00
Pages
3042-9
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC367038
Subset
IM
Grants
NIAID NIH HHS · AI20846 · United States
NCI NIH HHS · CA39612 · United States
NHLBI NIH HHS · HL33942 · United States
Databases
GENBANK
M13879
External Links
PubMed source DOI: 10.1128/mcb.6.9.3042-3049.1986 PMC Full Text View journal details
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