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PMID: 3491291 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Characterization of murine A-raf, a new oncogene related to the v-raf oncogene.

Molecular and cellular biology ·Vol. 6 ·No. 7 ·1986-07-00 ·Pages 2655-62

Huleihel M, Goldsborough M, Cleveland J, Gunnell M, Bonner T, Rapp UR

Abstract

A 1.6-kilobase cDNA (A-raf) has been isolated from a murine spleen cDNA library which encodes part of a protein related to the raf oncogene. Its amino acid sequence has 85% homology to raf in a central portion of 100 amino acids. In contrast to raf, A-raf shows a highly restricted tissue distribution of expression, with highest levels observed in epididymis, followed by intestine. When incorporated into a retrovirus, the resulting gag-A-raf fusion gene causes transformation in vitro and induces tumors in newborn mice. Thus, A-raf represents a new proto-oncogene. Transformation by A-raf is independent of ras gene function, as is the case for raf and mos but not other oncogenes.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Line DNA/isolation & purification Gene Expression Regulation Mice Oncogenes Spleen/analysis Tissue Distribution Viral Proteins/analysis
Chemicals
Viral Proteins DNA
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Huleihel M
Goldsborough M
Cleveland J
Gunnell M
Bonner T
Rapp U R
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38 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.6.7.2655-2662.1986
Published
1986-07-00
Pages
2655-62
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC367822
Subset
IM
Grants
NCI NIH HHS · N01-CO-23910 · United States
Databases
GENBANK
M13071
External Links
PubMed source DOI: 10.1128/mcb.6.7.2655-2662.1986 PMC Full Text View journal details
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