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PMID: 3481263 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Neutral endopeptidase-24.11 (enkephalinase). Biosynthesis and localization in human fibroblasts.

The Biochemical journal ·Vol. 248 ·No. 2 ·1987-12-01 ·Pages 345-50

Lorkowski G, Zijderhand-Bleekemolen JE, Erdös EG, von Figura K, Hasilik A

Abstract

The biosynthesis, glycosylation and subcellular localization of the neutral endopeptidase-24.11 were studied in cultured human fibroblasts. The enzyme was synthesized as a precursor (Mr 88,000) containing four or five N-linked oligosaccharides. Within 1 h the synthesis-mature (Mr 94,000) endopeptidase-24.11 was formed and contained sialylated oligosaccharides. The half-life of endopeptidase-24.11 was 3.7 days and in the presence of 10 mM-NH4Cl it increased to 6 days. Mature endopeptidase-24.11 was solubilized with 0.2% saponin and partitioned into Triton X-114. In intact fibroblasts, endopeptidase-24.11 was accessible to antibodies and to neuraminidase even when the treatment was performed at 4 degrees C. The localization of endopeptidase-24.11 to the plasma membrane in cultured fibroblasts was further demonstrated by immunocytochemistry.

MeSH Terms
Cell Membrane/enzymology Cells, Cultured Electrophoresis, Polyacrylamide Gel Fibroblasts/enzymology Glycosylation Humans Immunohistochemistry Metalloendopeptidases/biosynthesis,metabolism Neprilysin Subcellular Fractions/enzymology
Chemicals
Metalloendopeptidases Neprilysin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Lorkowski G
Institut für Physiologische Chemie und Pathobiochemie, Westfälische Wilhelms-Universität, Münster, Federal Republic of Germany.
Zijderhand-Bleekemolen J E
Erdös E G
von Figura K
Hasilik A
References (44)
44 references, click to expand
  1. Purification of endopeptidase-24.11 ('enkephalinase') from pig brain by immunoadsorbent chromatography.
    Biochem J. 1983 Dec 1;215(3):519-23 PMID: 6419725
  2. Metabolism of neuropeptides. Hydrolysis of the angiotensins, bradykinin, substance P and oxytocin by pig kidney microvillar membranes.
    Biochem J. 1987 Jan 1;241(1):237-47 PMID: 2436610
  3. Identification of a thermolysin-like metalloendopeptidase in serum: activity in normal subjects and in patients with sarcoidosis.
    J Lab Clin Med. 1984 Mar;103(3):420-31 PMID: 6366093
  4. Processing and transport of lysosomal enzymes in human monocyte line U937.
    Hoppe Seylers Z Physiol Chem. 1984 Aug;365(8):867-76 PMID: 6237973
  5. Hydrolysis of substance p and neurotensin by converting enzyme and neutral endopeptidase.
    Peptides. 1984 Jul-Aug;5(4):769-76 PMID: 6208535
  6. The metabolism of neuropeptides. The hydrolysis of peptides, including enkephalins, tachykinins and their analogues, by endopeptidase-24.11.
    Biochem J. 1984 Oct 15;223(2):433-40 PMID: 6149747
  7. Proteins of the kidney microvillar membrane. Biosynthesis of endopeptidase-24.11, dipeptidylpeptidase IV and aminopeptidases N and A in pig kidney slices.
    Biochem J. 1984 Dec 1;224(2):549-58 PMID: 6151393
  8. Mannose 6-phosphate-specific receptor is a transmembrane protein with a C-terminal extension oriented towards the cytosol.
    Biochem J. 1985 Jan 15;225(2):543-7 PMID: 3156583
  9. Neutral metalloendopeptidase in human male genital tract. Comparison to angiotensin I-converting enzyme.
    Lab Invest. 1985 Apr;52(4):437-47 PMID: 2984462
  10. An immunoradiometric assay for endopeptidase-24.11 shows it to be a widely distributed enzyme in pig tissues.
    Biochem J. 1985 May 15;228(1):119-26 PMID: 3890837
  11. Neutral metalloendopeptidase in human lung tissue and cultured cells.
    Am Rev Respir Dis. 1985 Sep;132(3):564-8 PMID: 2994530
  12. The metabolism of neuropeptides. Phase separation of synaptic membrane preparations with Triton X-114 reveals the presence of aminopeptidase N.
    Biochem J. 1985 Oct 15;231(2):445-9 PMID: 2865952
  13. Possible pathways for lysosomal enzyme delivery.
    J Cell Biol. 1985 Dec;101(6):2253-62 PMID: 2933416
  14. Neutral endopeptidase in serum samples from patients with adult respiratory distress syndrome. Comparison with angiotensin-converting enzyme.
    Am Rev Respir Dis. 1985 Dec;132(6):1262-7 PMID: 3000235
  15. Neutral endopeptidase 24.11 in human neutrophils: cleavage of chemotactic peptide.
    Proc Natl Acad Sci U S A. 1985 Dec;82(24):8737-41 PMID: 3909153
  16. Intracellular protein catabolism: state of the art.
    FEBS Lett. 1986 Mar 31;198(2):181-93 PMID: 3007210
  17. Ammonium chloride slows transport of the influenza virus hemagglutinin but does not cause mis-sorting in a polarized epithelial cell line.
    J Biol Chem. 1986 Nov 15;261(32):15172-8 PMID: 3771570
  18. Endopeptidase-24.11 in pig lymph nodes. Purification and immunocytochemical localization in reticular cells.
    Biochem J. 1986 Jun 15;236(3):801-10 PMID: 3539105
  19. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  20. The molecular weight and properties of a neutral metallo-endopeptidase from rabbit kidney brush border.
    Biochem J. 1974 Mar;137(3):489-95 PMID: 4214106
  21. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.
    Eur J Biochem. 1975 Aug 15;56(2):335-41 PMID: 1175627
  22. Establishment and characterization of a human histiocytic lymphoma cell line (U-937).
    Int J Cancer. 1976 May 15;17(5):565-77 PMID: 178611
  23. Continuous growth and differentiation of human myeloid leukaemic cells in suspension culture.
    Nature. 1977 Nov 24;270(5635):347-9 PMID: 271272
  24. An alternative hypothesis of cellular transport of lysosomal enzymes in fibroblasts. Effect of inhibitors of lysosomal enzyme endocytosis on intra- and extra-cellular lysosomal enzyme activities.
    Biochem J. 1978 Dec 15;176(3):943-50 PMID: 747663
  25. Inhibition of receptor-mediated uptake of a lysosomal enzyme into fibroblasts by chloroquine, procaine and ammonia.
    Exp Cell Res. 1979 Mar 15;119(2):359-64 PMID: 428466
  26. Biosynthesis of lysosomal enzymes in fibroblasts. Synthesis as precursors of higher molecular weight.
    J Biol Chem. 1980 May 25;255(10):4937-45 PMID: 6989821
  27. A neutral endopeptidase in the microvillar membrane of pig intestine. Partial purification and properties.
    Biochem J. 1980 Nov 1;191(2):645-8 PMID: 7016112
  28. Membrane bound pituitary metalloendopeptidase: apparent identity to enkephalinase.
    Biochem Biophys Res Commun. 1981 Sep 16;102(1):206-14 PMID: 7030340
  29. Oligosaccharides in lysosomal enzymes. Distribution of high-mannose and complex oligosaccharides in cathepsin D and beta-hexosaminidase.
    Eur J Biochem. 1981 Dec;121(1):125-9 PMID: 6459926
  30. Enhanced breakdown of arylsulfatase A in multiple sulfatase deficiency.
    Eur J Biochem. 1982 Apr 1;123(2):317-21 PMID: 6122572
  31. Lysosomotropic agents ammonium chloride and chloroquine inhibit both the synthesis and secretion of procollagen by freshly isolated embryonic chick tendon cells.
    Biochem Biophys Res Commun. 1982 Apr 14;105(3):902-8 PMID: 7092908
  32. Degradation of enkephalins: the search for an enkephalinase.
    Mol Cell Biochem. 1982 Aug 20;47(1):35-43 PMID: 6752692
  33. Biosynthesis and transport of cathepsin D in cultured human fibroblasts.
    J Cell Biol. 1983 Jul;97(1):1-5 PMID: 6863385
  34. Proteins of the kidney microvillar membrane. The amphipathic forms of endopeptidase purified from pig kidneys.
    Biochem J. 1983 Jun 1;211(3):743-53 PMID: 6349615
  35. Proteins of the kidney microvillar membrane. Reconstitution of endopeptidase in liposomes shows that it is a short-stalked protein.
    Biochem J. 1983 Jun 1;211(3):755-62 PMID: 6349616
  36. Human kidney "enkephalinase", a neutral metalloendopeptidase that cleaves active peptides.
    Biochemistry. 1983 Jun 21;22(13):3265-71 PMID: 6349683
  37. Juvenile and adult metachromatic leukodystrophy: partial restoration of arylsulfatase A (cerebroside sulfatase) activity by inhibitors of thiol proteinases.
    Proc Natl Acad Sci U S A. 1983 Oct;80(19):6066-70 PMID: 6136972
  38. A monoclonal antibody to kidney endopeptidase-24.11. Its application in immunoadsorbent purification of the enzyme and immunofluorescent microscopy of kidney and intestine.
    Biochem J. 1983 Aug 15;214(2):377-86 PMID: 6351851
  39. Heterogeneous distribution of enkephalin-degrading peptidases between neuronal and glial cells.
    Biochem Biophys Res Commun. 1983 Sep 15;115(2):423-9 PMID: 6626198
  40. Endopeptidase-24.11 purified from pig intestine is differently glycosylated from that in kidney.
    Biochem J. 1983 Nov 1;215(2):317-23 PMID: 6418142
  41. Anchoring of membrane proteins via phosphatidylinositol is deficient in two classes of Thy-1 negative mutant lymphoma cells.
    EMBO J. 1986 Dec 1;5(12):3291-6 PMID: 2880714
  42. An immunohistochemical study of endopeptidase-24.11 and aminopeptidase N in lymphoid tissues.
    Immunology. 1987 Feb;60(2):247-53 PMID: 3546102
  43. The N-terminal amino acid sequence of pig kidney endopeptidase-24.11 shows homology with pro-sucrase-isomaltase.
    Biochem J. 1986 Nov 15;240(1):305-8 PMID: 3548708
  44. Effects of exogenous amines on mammalian cells, with particular reference to membrane flow.
    Biochem J. 1984 Jan 1;217(1):27-40 PMID: 6365083
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2480345
Published
1987-12-01
Pages
345-50
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1148547
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2480345 PMC Full Text View journal details
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