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PMID: 3475717 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Changes in neural cell adhesion molecule (NCAM) structure during vertebrate neural development.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 16 ·1987-08-00 ·Pages 5986-90

Sunshine J, Balak K, Rutishauser U, Jacobson M

Abstract

Changes in carbohydrate and polypeptide form of the neural cell adhesion molecule (NCAM) have been documented during the development of central nervous system tissue in both chicken and frog. The carbohydrate variations reflect a high and low content of polysialic acid, and for the two vertebrates examined the expression of these forms is similar. At very early stages of neural development NCAM with a low content of polysialic acid is present, during histogenesis of the central nervous system NCAM with a high content of polysialic acid dominates, and there is a gradual return to NCAM with a low content of polysialic acid as the animals approach maturity. In contrast, the order of expression of the major NCAM polypeptide forms is different in the chicken and frog. These findings suggest that changes in sialic acid are a fundamental aspect of the function of NCAM in development, whereas NCAM polypeptide differences may affect events associated with a particular vertebrate. Studies have demonstrated that a decreased sialic acid content enhances the adhesion properties of NCAM. On this basis, we propose that NCAM with a low content of polysialic acid functions both to maintain integrity of neuroepithelium during morphogenesis of the early embryo and to stabilize differentiated structures in the adult, while the decreased adhesive function of NCAM with a high content of polysialic acid provides more plasticity in cell interactions during cell migration, axon outgrowth, and formation of neural circuits.

MeSH Terms
Animals Antigens, Surface/analysis Carbohydrates/analysis Cell Adhesion Molecules Central Nervous System/growth & development Chick Embryo Electrophoresis, Polyacrylamide Gel Peptides/analysis Xenopus laevis
Chemicals
Antigens, Surface Carbohydrates Cell Adhesion Molecules Peptides
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Sunshine J
Balak K
Rutishauser U
Jacobson M
References (42)
42 references, click to expand
  1. Effects of fasciculation on the outgrowth of neurites from spinal ganglia in culture.
    J Cell Biol. 1980 NOV;87(2 Pt 1):370-8 PMID: 7430247
  2. Expression of Xenopus N-CAM RNA in ectoderm is an early response to neural induction.
    Development. 1987 Mar;99(3):311-25 PMID: 2443340
  3. A dot-immunobinding assay for monoclonal and other antibodies.
    Anal Biochem. 1982 Jan 1;119(1):142-7 PMID: 7072935
  4. Chemical characterization of a neural cell adhesion molecule purified from embryonic brain membranes.
    J Biol Chem. 1982 Jul 10;257(13):7720-9 PMID: 7085646
  5. Molecular heterogeneity and structural evolution during cerebellar ontogeny detected by monoclonal antibody of the mouse cell surface antigen BSP-2.
    Brain Res. 1983 Apr 11;265(1):87-100 PMID: 6189553
  6. Differences in the carbohydrate structures of neural cell-adhesion molecules from adult and embryonic chicken brains.
    J Biol Chem. 1982 Sep 25;257(18):11064-9 PMID: 6809766
  7. Monoclonal antibodies as neural cell surface markers.
    Neurochem Res. 1982 Mar;7(3):349-62 PMID: 7050755
  8. Cell adhesion molecules in early chicken embryogenesis.
    Proc Natl Acad Sci U S A. 1982 Nov;79(21):6737-41 PMID: 6959151
  9. Embryonic to adult conversion of neural cell adhesion molecules in normal and staggerer mice.
    Proc Natl Acad Sci U S A. 1982 Nov;79(22):7036-40 PMID: 6960362
  10. Clonal organization of the central nervous system of the frog. III. Clones stemming from individual blastomeres of the 128-, 256-, and 512-cell stages.
    J Neurosci. 1983 May;3(5):1019-38 PMID: 6842280
  11. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  12. Adhesion among neural cells of the chick embryo. III. Relationship of the surface molecule CAM to cell adhesion and the development of histotypic patterns.
    J Cell Biol. 1978 Nov;79(2 Pt 1):371-81 PMID: 569155
  13. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
  14. Neural cell adhesion molecule mediates initial interactions between spinal cord neurons and muscle cells in culture.
    J Cell Biol. 1983 Jul;97(1):145-52 PMID: 6863388
  15. Adult and embryonic mouse neural cell adhesion molecules have different binding properties.
    Nature. 1983 Jul 28-Aug 3;304(5924):347-9 PMID: 6877355
  16. Kinetics of homophilic binding by embryonic and adult forms of the neural cell adhesion molecule.
    Proc Natl Acad Sci U S A. 1983 Sep;80(18):5762-6 PMID: 6577452
  17. Fiber-fiber interaction and tectal cues influence the development of the chicken retinotectal projection.
    Proc Natl Acad Sci U S A. 1984 Mar;81(6):1906-10 PMID: 6584925
  18. Use of prokaryotic-derived probes to identify poly(sialic acid) in neonatal neuronal membranes.
    Proc Natl Acad Sci U S A. 1984 Apr;81(7):1971-5 PMID: 6371806
  19. Tissue- and developmental stage-specific forms of a neural cell surface antigen linked to differences in glycosylation of a common polypeptide.
    EMBO J. 1982;1(10):1239-44 PMID: 7188249
  20. Topological distribution of different forms of neural cell adhesion molecule in the developing chick visual system.
    Nature. 1984 Jul 12-18;310(5973):141-3 PMID: 6204209
  21. Studies on the transmembrane disposition of the neural cell adhesion molecule N-CAM. A monoclonal antibody recognizing a cytoplasmic domain and evidence for the presence of phosphoserine residues.
    Eur J Biochem. 1984 Jul 2;142(1):57-64 PMID: 6745267
  22. Studies on the transmembrane disposition of the neural cell adhesion molecule N-CAM. The use of liposome-inserted radioiodinated N-CAM to study its transbilayer orientation.
    Eur J Biochem. 1984 Jul 2;142(1):65-73 PMID: 6745268
  23. Alterations in neural cell adhesion molecules during development of different regions of the nervous system.
    J Neurosci. 1984 Sep;4(9):2354-68 PMID: 6481452
  24. Evolutionary conservation of key structures and binding functions of neural cell adhesion molecules.
    Proc Natl Acad Sci U S A. 1984 Nov;81(21):6881-5 PMID: 6208560
  25. Guidance of optic axons in vivo by a preformed adhesive pathway on neuroepithelial endfeet.
    Dev Biol. 1984 Dec;106(2):485-99 PMID: 6500184
  26. Cleavage of the polysialosyl units of brain glycoproteins by a bacteriophage endosialidase. Involvement of a long oligosaccharide segment in molecular interactions of polysialic acid.
    J Biol Chem. 1985 Jan 25;260(2):1265-70 PMID: 3968060
  27. Involvement of the nerve growth factor-inducible large external glycoprotein (NILE) in neurite fasciculation in primary cultures of rat brain.
    Proc Natl Acad Sci U S A. 1985 Feb;82(4):1276-80 PMID: 3856258
  28. Phylogeny of a neural cell adhesion molecule.
    Dev Biol. 1985 Jul;110(1):39-46 PMID: 2408943
  29. Neural cell adhesion molecule (N-CAM) accumulates in denervated and paralyzed skeletal muscles.
    Proc Natl Acad Sci U S A. 1985 Jul;82(13):4544-8 PMID: 3892537
  30. Glial cells express N-CAM/D2-CAM-like polypeptides in vitro.
    Nature. 1985 Aug 22-28;316(6030):725-8 PMID: 2412126
  31. Specific alteration of NCAM-mediated cell adhesion by an endoneuraminidase.
    J Cell Biol. 1985 Nov;101(5 Pt 1):1842-9 PMID: 4055897
  32. Selective expression of the 180-kD component of the neural cell adhesion molecule N-CAM during development.
    J Cell Biol. 1985 Nov;101(5 Pt 1):1921-9 PMID: 3902857
  33. Biosynthesis of the neural cell adhesion molecule: characterization of polypeptide C.
    J Cell Biol. 1985 Dec;101(6):2310-5 PMID: 4066759
  34. Sequence of a cDNA clone encoding the polysialic acid-rich and cytoplasmic domains of the neural cell adhesion molecule N-CAM.
    Proc Natl Acad Sci U S A. 1986 May;83(9):3037-41 PMID: 3458261
  35. Induction of neural cell adhesion molecule (NCAM) in Xenopus embryos.
    Dev Biol. 1986 Aug;116(2):524-31 PMID: 3525283
  36. Differential expression of mouse neural cell-adhesion molecule (N-CAM) mRNA species during brain development and in neural cell lines.
    J Neurosci. 1986 Jul;6(7):1983-90 PMID: 3734870
  37. Developmental study of detergent solubility and polypeptide composition of the neural cell adhesion molecule.
    Dev Neurosci. 1986;8(1):24-30 PMID: 3743467
  38. Topography of N-CAM structural and functional determinants. I. Classification of monoclonal antibody epitopes.
    J Cell Biol. 1986 Nov;103(5):1721-7 PMID: 2430976
  39. Topography of N-CAM structural and functional determinants. II. Placement of monoclonal antibody epitopes.
    J Cell Biol. 1986 Nov;103(5):1729-37 PMID: 2430977
  40. Neural cell adhesion molecule expression in Xenopus embryos.
    Dev Biol. 1987 Feb;119(2):540-50 PMID: 3542639
  41. Visualization of neural cell adhesion molecule by electron microscopy.
    J Cell Biol. 1987 Jun;104(6):1579-86 PMID: 2438287
  42. Neural cell adhesion molecule is on embryonic muscle cells and mediates adhesion to nerve cells in vitro.
    Nature. 1982 Feb 25;295(5851):693-5 PMID: 6173773
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.16.5986
Published
1987-08-00
Pages
5986-90
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC298988
Subset
IM
Grants
NEI NIH HHS · EY 06107 · United States
NICHD NIH HHS · HD 07104 · United States
NICHD NIH HHS · HD 18369 · United States
External Links
PubMed source DOI: 10.1073/pnas.84.16.5986 PMC Full Text View journal details
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