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PMID: 3472242 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Vaccines containing purified GM2 ganglioside elicit GM2 antibodies in melanoma patients.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 9 ·1987-05-00 ·Pages 2911-5

Livingston PO, Natoli EJ, Calves MJ, Stockert E, Oettgen HF, Old LJ

Abstract

GM2, GD2, and GD3 gangliosides are expressed on the surface of human melanoma cells and represent potential targets for immunological control of melanoma growth by monoclonal antibodies and active immunization. The immunogenicity of GM2 was investigated by analyzing the humoral immune response of melanoma patients to vaccination with cell lines selected for high GM2 expression and with vaccines containing purified GM2. The whole-cell vaccine and vaccines containing purified GM2 and bacillus Calmette-Guérin (BCG) elicited GM2 antibody in a high proportion of patients, particularly in GM2/BCG-vaccinated patients pretreated with cyclophosphamide and given a GM2/BCG booster immunization. Vaccines containing purified GM2 and Salmonella minnesota R595 as the adjuvant were also effective, but only in patients pretreated with cyclophosphamide. GM2 antibodies in vaccinated patients were of the IgM class and were cytotoxic for GM2-positive targets in the presence of human complement.

MeSH Terms
Animals Antibody Formation Cell Line G(M2) Ganglioside/immunology Gangliosides/analysis,immunology Humans Melanoma/immunology Melanoma, Experimental/immunology Mice Vaccines/immunology
Chemicals
Gangliosides Vaccines G(M2) Ganglioside
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Livingston P O
Natoli E J
Calves M J
Stockert E
Oettgen H F
Old L J
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29 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.9.2911
Published
1987-05-00
Pages
2911-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC304770
Subset
IM
Grants
NCI NIH HHS · CA-08748 · United States
NCI NIH HHS · CA-36120 · United States
External Links
PubMed source DOI: 10.1073/pnas.84.9.2911 PMC Full Text View journal details
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