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PMID: 6193515 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Ganglioside GM2 as a human tumor antigen (OFA-I-1).

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 17 ·1983-09-00 ·Pages 5392-6

Tai T, Paulson JC, Cahan LD, Irie RF

Abstract

A monospecific antibody produced in vitro by a B-lymphoblastoid cell line transformed with Epstein-Barr virus has been shown to recognize a membrane antigen (OFA-I-1) on human tumors and fetal brain. This study identifies the chemical nature of OFA-I-1. The glycolipid fraction of antigen-rich spent medium of an OFA-I-1-positive melanoma cell line, M14, was extracted by chloroform/methanol/water, 4:8:3 (vol/vol), and was separated into fractions of neutral glycolipids and gangliosides by DEAE-Sephadex followed by base treatment and Bio-sil A column elution. OFA-I-1 antigens were found exclusively in the ganglioside fraction when assayed with monospecific anti-OFA-I-1 by an immune adherence inhibition test. The results obtained from thin-layer chromatography of the antigenic M14 ganglioside and sequential glycosidase digestion suggested that the antigen was a ganglioside GM2, GalNAc beta 1 leads to 4(NeuAc alpha 2 leads to 3)Gal beta 1 leads to 4Glc leads to Cer. These results were further supported by testing various authentic gangliosides and neutral glycolipids for OFA-I-1 antigenicity. Only GM2 showed positive reactivity.

MeSH Terms
Adult Antigens, Neoplasm/immunology Brain/immunology Carbohydrates/analysis Cell Line Epitopes/analysis Female Fetus G(M2) Ganglioside/immunology Gangliosides/immunology,isolation & purification Glycolipids/isolation & purification Humans Liver/immunology Melanoma/immunology Pregnancy
Chemicals
Antigens, Neoplasm Carbohydrates Epitopes Gangliosides Glycolipids oncofetal antigens G(M2) Ganglioside
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Tai T
Paulson J C
Cahan L D
Irie R F
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26 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.17.5392
Published
1983-09-00
Pages
5392-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC384262
Subset
IM
Grants
NCI NIH HHS · CA00543 · United States
NCI NIH HHS · CA12582 · United States
NCI NIH HHS · CA30647 · United States
External Links
PubMed source DOI: 10.1073/pnas.80.17.5392 PMC Full Text View journal details
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