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PMID: 3467722 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Isolation and characterization of the M.EaeI modification methylase.

The Biochemical journal ·Vol. 238 ·No. 2 ·1986-09-01 ·Pages 613-5

Jacobs D, Brown NL

Abstract

The modification enzyme (M.EaeI) corresponding to the restriction endonuclease EaeI was partially purified from Enterobacter aerogenes PW201. The M.EaeI enzyme methylates the innermost cytosine residue in each strand of the family of related sequences that constitute the EaeI recognition site to give: 5'-Y-G-G-5mC-C-R-3' where 5mC is 5-methylcytosine. M.EaeI protects these sites against cleavage by HaeIII, and protects overlapping 5'-C-C-G-G-3' sites against cleavage by both HpaII and MspI.

MeSH Terms
Binding Sites Chromatography, Paper DNA/metabolism Electrophoresis, Polyacrylamide Gel Enterobacter/enzymology Enterobacteriaceae/enzymology Methylation Methyltransferases/isolation & purification,metabolism Site-Specific DNA-Methyltransferase (Adenine-Specific)
Chemicals
DNA Methyltransferases Site-Specific DNA-Methyltransferase (Adenine-Specific)
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Jacobs D
Brown N L
References (11)
11 references, click to expand
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    Nucleic Acids Res. 1977 Dec;4(12):4211-21 PMID: 600794
  2. The nucleotide sequence of bacteriophage phiX174.
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  3. Complete nucleotide sequence of the Escherichia coli plasmid pBR322.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:77-90 PMID: 383387
  4. AhaIII: a restriction endonuclease with a recognition sequence containing only A:T basepairs.
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  6. Effect of CpG methylation on Msp I.
    Nucleic Acids Res. 1983 Jun 11;11(11):3571-80 PMID: 6190136
  7. Cytosine modification in DNA by BcnI methylase yields N4-methylcytosine.
    FEBS Lett. 1983 Sep 5;161(1):131-4 PMID: 6884523
  8. Site-specific cleavage of DNA at 8- and 10-base-pair sequences.
    Proc Natl Acad Sci U S A. 1984 Feb;81(4):983-7 PMID: 6322193
  9. Restriction and modification enzymes and their recognition sequences.
    Nucleic Acids Res. 1985;13 Suppl:r165-200 PMID: 2987885
  10. The effect of site specific methylation on restriction endonuclease digestion.
    Nucleic Acids Res. 1985;13 Suppl:r201-7 PMID: 2987886
  11. Analysis of products of DNA modification by methylases: a procedure for the determination of 5- and N4-methylcytosines in DNA.
    Anal Biochem. 1985 Jul;148(1):194-8 PMID: 2994519
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2380613
Published
1986-09-01
Pages
613-5
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1147176
Subset
IM
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1042/bj2380613 PMC Full Text View journal details
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