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PMID: 345968 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Restriction endonuclease analysis for the identification of baculovirus pesticides.

Applied and environmental microbiology ·Vol. 35 ·No. 2 ·1978-02-00 ·Pages 411-21

Miller LK, Dawes KP

Abstract

Gel electrophoresis of deoxyribonucleic acid (DNA) fragments generated by digesting the DNA genomes of nuclear polyhedrosis viruses (NPV) with restriction endonucleases provides DNA fragment patterns that may be used to identify different viruses of this group. Characteristic fragment patterns were obtained for three NPVs, which are important as biological pesticides (Autographa californica NPV, Orgyia pseudotsugata NPV, and Heliothis zea NPV). The DNA fragment patterns of the A. californica NPV genoms did not change with passage through the alternate insect host, Trichoplusia ni. Heterogeneity in one preparation of O. pseudotsugata NPV was observed. The identification procedure is direct and precise. Applications of this procedure include quality control of commercial preparations of viral pesticides and screening for genetic alterations in the viruses.

MeSH Terms
DNA Restriction Enzymes DNA, Viral/analysis Electrophoresis, Agar Gel Genes, Viral Insect Viruses/analysis,genetics Methods
Chemicals
DNA, Viral DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Miller L K
Dawes K P
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23 references, click to expand
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/aem.35.2.411-421.1978
Published
1978-02-00
Pages
411-21
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC242846
Subset
IM
External Links
PubMed source DOI: 10.1128/aem.35.2.411-421.1978 PMC Full Text View journal details
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