Effect of mutations affecting lysyl-tRNAlys on the regulation of lysine biosynthesis in Escherichia coli.
When studying mutants affecting lysyl-tRNA synthetase or tRNAlys (hisT, hisW), a lack of correlation is clearly observed between the amount of lysyl-tRNA and the level of derepression of several lysine biosynthetic enzymes. This exlcudes the possible role of lysyl-tRNA as the specific corepressor of the lysine regulon. However, the level of derepression of DAP-decarboxylase, the last enzyme of the lysine pathway, is very low in the hisT mutant; this indicates that tRNAlys is a secondary effector involved in the regulation of the synthesis of this enzyme.
18 references, click to expand
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Characterization of a cold-sensitive hisW mutant of Salmonella typhimurium.J Bacteriol. 1973 May;114(2):528-36 PMID: 4350342
-
Repression of the tyrosine, lysine, and methionine biosynthetic pathways in a hisT mutant of Salmonella typhimurium.J Bacteriol. 1977 Feb;129(2):1168-70 PMID: 14106
-
The regulation of diaminopimelate decarboxylase activity in Escherichia coli strain w.J Gen Microbiol. 1976 Sep;96(1):51-62 PMID: 789815
-
Biochemical and regulatory properties of Escherichia coli K-12 hisT mutants.J Bacteriol. 1977 Apr;130(1):4-10 PMID: 323237
-
An in vivo effect of the metabolites L-alanine and glycyl-L-leucine on the properties of lysyl-tRNA synthetase from Escherichia coli K-12. I. Influence on subunit composition and molecular weight distribution.Biochim Biophys Acta. 1976 Jul 2;435(3):290-305 PMID: 779846
-
Dual-control of the tryptophan operon is mediated by both tryptophanyl-tRNA synthetase and the repressor.J Mol Biol. 1976 May 15;103(2):209-26 PMID: 781267
-
Metabolites influence control of lysine transfer ribonucleic acid synthetase formation in Escherichia coli K-12.Proc Natl Acad Sci U S A. 1975 Apr;72(4):1364-7 PMID: 805427
-
[Repression of meso-alpha, epsilon-diaminopimelic acid decarboxylase by L-lysine in Escherichia coli].Biochim Biophys Acta. 1962 Apr 9;58:359-60 PMID: 14484389
-
[Mutant tRNA His ineffective in repression and lacking two pseudouridine modifications].Nat New Biol. 1972 Jul 19;238(81):72-4 PMID: 4558263
-
Reduced maximal levels of derepression of the isoleucine-valine and leucine enzymes in hisT mutants of Salmonella typhimurium.Nature. 1975 Jan 24;253(5489):279-80 PMID: 1089896
-
Threonyl-transfer ribonucleic acid synthetase and the regulation of the threonine operon in Escherichia coli.J Bacteriol. 1977 Jan;129(1):66-70 PMID: 318654
-
On the role of isoleucyl-tRNA synthetase in multivalent repression.Biochem Genet. 1972 Apr;6(2):99-118 PMID: 4581142
-
Role of lysyl-tRNA in the regulation of lysine biosynthesis in Escherichia coli K12.Biochimie. 1976;58(1-2):213-8 PMID: 8152
-
Histidine regulation in Salmonella typhimurium: an activator attenuator model of gene regulation.Proc Natl Acad Sci U S A. 1975 Sep;72(9):3453-7 PMID: 1103149
-
Transcription termination in vivo in the leader region of the tryptophan operon of Escherichia coli.J Mol Biol. 1976 May 15;103(2):319-37 PMID: 781269
-
Histidine regulation in Salmonella typhimurium. XI. The percentage of transfer RNA His charged in vivo and its relation to the repression of the histidine operon.J Mol Biol. 1972 Apr 28;66(1):131-42 PMID: 4339187
-
Biosynthesis of pseudouridine in transfer ribonucleic acid.J Biol Chem. 1974 Feb 25;249(4):1103-8 PMID: 4592259
-
Pleiotropy of hisT mutants blocked in pseudouridine synthesis in tRNA: leucine and isoleucine-valine operons.Proc Natl Acad Sci U S A. 1974 May;71(5):1857-61 PMID: 4151955
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