Fast purification of a functional elongator tRNAmet expressed from a synthetic gene in vivo.

Nucleic acids research ·Vol. 16 ·No. 16 ·1988-08-25 ·Pages 8095-6

Meinnel T, Mechulam Y, Fayat G

Abstract

暂无摘要

MeSH Terms

Base Sequence Genes, Synthetic Molecular Sequence Data Peptide Elongation Factors/genetics,isolation & purification RNA, Transfer, Amino Acid-Specific/isolation & purification RNA, Transfer, Met/genetics,isolation & purification

Chemicals

Peptide Elongation Factors RNA, Transfer, Amino Acid-Specific RNA, Transfer, Met

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Meinnel T

Ecole Polytechnique, UA no. 240, CNRS, Palaiseau, France.

Mechulam Y

Fayat G

References (4)

4 references, click to expand

The mechanism of action of methionyl-tRNA synthetase from Escherichia coli. 1. Fluorescence studies on tRNAMet binding as a function of ligands, ions and pH.
Eur J Biochem. 1973 Jul 2;36(1):213-26 PMID: 4581817
Effect of the overproduction of phenylalanyl- and threonyl-tRNA synthetases on tRNAPhe and tRNAThr concentrations in E. coli cells.
Biochimie. 1983 Mar;65(3):221-5 PMID: 6405814
Construction of two Escherichia coli amber suppressor genes: tRNAPheCUA and tRNACysCUA.
Proc Natl Acad Sci U S A. 1986 Sep;83(17):6548-52 PMID: 3529087
Computer simulation of DNA ligation: determination of initial DNA concentrations favouring the formation of recombinant molecules.
Nucleic Acids Res. 1988 Mar 11;16(5):1767-78 PMID: 2832827

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/16.16.8095

Published

1988-08-25

Pages

8095-6

Language

English

Region

England

NLM ID

0411011

PMCID

PMC338511

Subset

External Links

PubMed source DOI: 10.1093/nar/16.16.8095 PMC Full Text View journal details

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Fast purification of a functional elongator tRNAmet expressed from a synthetic gene in vivo.

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