The mechanism of action of methionyl-tRNA synthetase from Escherichia coli. 1. Fluorescence studies on tRNAMet binding as a function of ligands, ions and pH.

Amino Acyl-tRNA Synthetases/antagonists & inhibitors,isolation & purification,metabolism Binding Sites Escherichia coli/enzymology Hydrogen-Ion Concentration Kinetics Magnesium Methionine Potassium Chloride Protein Binding Protein Conformation RNA, Transfer/isolation & purification,metabolism Spectrometry, Fluorescence Spermidine Spermine Trypsin

Spermine Potassium Chloride RNA, Transfer Methionine Trypsin Amino Acyl-tRNA Synthetases Magnesium Spermidine