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PMID: 341085 Published · ppublish English Journal Article

Genetically determined differences in concentrations of isoaccepting tRNAs in Escherichia coli.

Nucleic acids research ·Vol. 4 ·No. 12 ·1977-12-00 ·Pages 4313-22

Thomale J, Nass G

Abstract

Two examples of genetically determined altered concentrations of isoaccepting tRNAs are presented. The concentrations of isoaccepting tRNAsThr are selectively changed by a mutation causing a fourfold overproduction of the cognate aminoacyl-tRNA-synthetase, the threonyl-tRNA synthetase, whereas the distribution of isoaccepting tRNAs of four control tRNA-species in these E. coli mutants was not affected by that mutation. Secondly evidence is presented for a correlation between mutations in structural genes of aminoacid biosynthetic enzymes and alterations in concentrations of cognate isoaccepting tRNAs in two different E. coli strains, auxotrophic for threonine, isoleucine/valine and leucine, and arginine respectively.

MeSH Terms
Arginine Escherichia coli/genetics,metabolism Isoleucine Leucine RNA, Transfer/genetics,metabolism Species Specificity Threonine
Chemicals
Isoleucine Threonine RNA, Transfer Arginine Leucine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Thomale J
Nass G
References (29)
29 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/4.12.4313
Published
1977-12-00
Pages
4313-22
Language
English
Region
England
NLM ID
0411011
PMCID
PMC343243
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/4.12.4313 PMC Full Text View journal details
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