Derepressed levels of the isoleucine-valine and leucine enzymes in his T 1504, a strain of Salmonella typhimurium with altered leucine transfer ribonucleic acid.

Journal of bacteriology ·Vol. 117 ·No. 2 ·1974-02-00 ·Pages 449-55

Rizzino AA, Bresalier RS, Freundlich M

Abstract

A hisT mutant of Salmonella typhimurium was found to have altered regulation of the isoleucine-valine and leucine enzymes. These enzymes in the hisT strain were derepressed two- to eightfold over those of the parent wild-type strain when grown in minimal medium or under repressing conditions. The amount of tRNA(Leu) and the cellular concentration of charged tRNA(Leu) was about the same in the hisT strain and in the wild type. However, leucyl-tRNA from the mutant was chromatographically different from that of wild type, confirming previous reports that hisT strains have altered tRNA(Leu). These results suggest strongly that tRNA(Leu) is involved in repression of the isoleucine-valine and leucine enzymes in S. typhimurium.

MeSH Terms

Carbon Radioisotopes Cell-Free System Chromatography Enzyme Repression Histidine/biosynthesis Hydro-Lyases/metabolism Isoleucine/biosynthesis Leucine/biosynthesis Mutation Oxidoreductases/metabolism Oxo-Acid-Lyases/metabolism Periodic Acid RNA, Transfer/analysis,metabolism Salmonella typhimurium/enzymology,growth & development,metabolism Transaminases/metabolism Tritium Valine/biosynthesis

Chemicals

Carbon Radioisotopes Isoleucine Tritium Periodic Acid Histidine RNA, Transfer Oxidoreductases Transaminases Oxo-Acid-Lyases Hydro-Lyases Leucine Valine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Rizzino A A

Bresalier R S

Freundlich M

References (22)

22 references, click to expand

Columns for rapid chromatographic separation of small amounts of tracer-labeled transfer ribonucleic acids.
Anal Biochem. 1971 Dec;44(2):486-95 PMID: 4943341
Evidence that the majority of leucine transfer ribonucleic acid is not involved in repression in Salmonella typhimurium.
J Bacteriol. 1971 Nov;108(2):951-3 PMID: 4942773
Specific binding of leucyl transfer RNA to an immature form of L-threonine deaminase: its implications in repression.
Proc Natl Acad Sci U S A. 1970 Aug;66(4):1027-35 PMID: 4920088
Mutants of Escherichia coli requiring methionine or vitamin B12.
J Bacteriol. 1950 Jul;60(1):17-28 PMID: 15436457
Regulation of branched-chain amino acid biosynthesis in Salmonella typhimurium: isolation of regulatory mutants.
J Bacteriol. 1969 Mar;97(3):1272-82 PMID: 4887507
Histidine regulation in Salmonella typhimurium. IX. Histidine transfer ribonucleic acid of the regulatory mutants.
J Biol Chem. 1972 Feb 25;247(4):1080-8 PMID: 4551510
Purification and properties of the acetohydroxy acid isomeroreductase of Salmonella typhimurium.
J Biol Chem. 1969 Mar 10;244(5):1118-27 PMID: 4388025
Threonine deamination in Escherichia coli. II. Evidence for two L-threonine deaminases.
J Bacteriol. 1957 Jan;73(1):105-12 PMID: 13405870
The isolation and sequence analysis of transfer RNA: the use of plaskon chromatography (RPC-5).
Biochim Biophys Acta. 1973 Aug 10;319(1):25-36 PMID: 4582141
Inhibition of growth of Salmonella typhimurium and of threonine deaminase and transaminase B by beta-chloroalanine.
J Bacteriol. 1971 Feb;105(2):519-22 PMID: 5541528
Effect of cyclopentaneglycine on metabolism in Salmonella typhimurium.
J Bacteriol. 1972 Aug;111(2):510-5 PMID: 4559733
Isoleucine auxotrophy as a consequence of a mutationally altered isoleucyl-transfer ribonucleic acid synthetase.
J Bacteriol. 1971 Feb;105(2):527-37 PMID: 5541530
[Mutant tRNA His ineffective in repression and lacking two pseudouridine modifications].
Nat New Biol. 1972 Jul 19;238(81):72-4 PMID: 4558263
Histidine regulatory mutants in Salmonella typhimurium. I. Isolation and general properties.
J Mol Biol. 1966 Dec 28;22(2):305-23 PMID: 5339687
Control of isoleucine, valine, and leucine biosynthesis. I. Multivalent repression.
Proc Natl Acad Sci U S A. 1962 Oct 15;48:1804-8 PMID: 13959618
Leucine tRNA(1) from HisT mutant of Salmonella typhimurium lacks two pseudouridines.
FEBS Lett. 1972 Dec 1;28(2):205-208 PMID: 11946859
Biochemical and genetic characterization of a mutant of Escherichia coli with a temperature-sensitive valyl ribonucleic acid synthetase.
J Bacteriol. 1966 Oct;92(4):1076-82 PMID: 5333025
Mutants of Salmonella typhimurium with an altered leucyl-transfer ribonucleic acid synthetase.
J Bacteriol. 1971 Apr;106(1):213-20 PMID: 4928008
On the role of isoleucyl-tRNA synthetase in multivalent repression.
Biochem Genet. 1972 Apr;6(2):99-118 PMID: 4581142
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
Metabolite analogs as genetic and biochemical probes.
Adv Genet. 1971;16:119-40 PMID: 5004084
Control of isoleucine, valine and leucine biosynthesis. V. Dual effect of alpha-aminobutyric acid on repression and endproduct inhibition in Escherichia coli.
Biochim Biophys Acta. 1968 Dec 23;170(2):271-81 PMID: 4884572

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.117.2.449-455.1974

Published

1974-02-00

Pages

449-55

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC285533

Subset

External Links

PubMed source DOI: 10.1128/jb.117.2.449-455.1974 PMC Full Text View journal details

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Derepressed levels of the isoleucine-valine and leucine enzymes in his T 1504, a strain of Salmonella typhimurium with altered leucine transfer ribonucleic acid.

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