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PMID: 340897 Published · ppublish English Comparative Study Journal Article

Effects of chloramphenicol and caffeine on postreplication repair in uvr A- umuC- und uvrA- recF- strains of Escherichia coli K-12.

Molecular & general genetics : MGG ·Vol. 156 ·No. 2 ·1977-11-14 ·Pages 115-20

Kato T

Abstract

Postreplication repair and its inhibition by chloramphenicol and caffeine, as seen in alkaline sucrose gradients, were compared between a uv non-mutable strain uvrA- umuC- and normally mutable strains uvrA- recF- and uvrA- umu+rec+ of Escherichia coli K-12. The uvrA- umuC- strain performed postreplication repair as efficiently as the parental strain, while the repair in uvrA- recF- strain was dependent on UV dose. Both chloramphenicol and caffeine inhibited postreplication repair to an equal extent of about 25%, and 10%, respectively, in all three uvrA- strains of umuC36, recF- and umu+rec+. These observations suggest that postreplication repair is largely not responsible for UV mutagenesis.

MeSH Terms
Caffeine/pharmacology Chloramphenicol/pharmacology DNA Repair/drug effects DNA Replication Escherichia coli/genetics,radiation effects Mutation Phenotype Species Specificity Time Factors Ultraviolet Rays
Chemicals
Caffeine Chloramphenicol
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Kato T
References (21)
21 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00283483
Published
1977-11-14
Pages
115-20
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00283483
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